Functional Analysis of Human Hepatocytes Isolated From Chimeric Mouse Liver

N. Harimoto, H. Nakagawara, K. Shirabe, T. Yoshizumi, S. Itoh, T. Ikegami, Y. Soejima, Y. Maehara, Y. Ishida, C. Tateno, Y. Tanaka

研究成果: ジャーナルへの寄稿記事

抜粋

Chimeric mice with humanized liver were first established by transplanting primary human hepatocytes (PHHs) isolated from a Japanese 27-year-old donor into complementary DNA-urokinase-type plasminogen activator/severe combined immunodeficiency mice. The PHHs from the Japanese donor increased more than 100-fold in the mouse liver, and human hepatocytes purified from the chimeric mouse liver (hcPHs) were successfully transplanted into second-passaged mice. These PHHs and hcPHs can produce human albumin and preserve many liver-specific enzyme genes, which are important for liver function. Interestingly, hepatitis B virus can be infected with these chimeric mice; hepatitis B viral DNA and hepatitis B surface antigen levels were detectable. In conclusion, hcPHs can be an ideal cell source for analysis of human hepatocytes.

元の言語英語
ページ(範囲)3858-3862
ページ数5
ジャーナルTransplantation Proceedings
50
発行部数10
DOI
出版物ステータス出版済み - 12 2018

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

これを引用