Functional analysis of membrane-bound complement regulatory protein on T-cell immune response in ginbuna crucian carp

Indriyani Nur, Nevien K. Abdelkhalek, Shiori Motobe, Ryota Nakamura, Masakazu Tsujikura, Tomonori Somamoto, Miki Nakao

研究成果: ジャーナルへの寄稿記事

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Complements have long been considered to be a pivotal component in innate immunity. Recent researches, however, highlight novel roles of complements in T-cell-mediated adaptive immunity. Membrane-bound complement regulatory protein CD46, a costimulatory protein for T cells, is a key molecule for T-cell immunomodulation. Teleost CD46-like molecule, termed Tecrem, has been newly identified in common carp and shown to function as a complement regulator. However, it remains unclear whether Tecrem is involved in T-cell immune response. We investigated Tecrem function related to T-cell responses in ginbuna crucian carp. Ginbuna Tecrem (gTecrem) proteins were detected by immunoprecipitation using anti-common carp Tecrem monoclonal antibody (mAb) and were ubiquitously expressed on blood cells including CD8α+ and CD4+ lymphocytes. gTecrem expression on leucocyte surface was enhanced after stimulation with the T-cell mitogen, phytohaemagglutinin (PHA). Coculture with the anti-Tecrem mAb significantly inhibited the proliferative activity of PHA-stimulated peripheral blood lymphocytes, suggesting that cross-linking of Tecrems on T-cells interferes with a signal transduction pathway for T-cell activation. These findings indicate that Tecrem may act as a T-cell moderator and imply that the complement system in teleost, as well as mammals, plays an important role for linking adaptive and innate immunity.

元の言語英語
ページ(範囲)1-7
ページ数7
ジャーナルMolecular Immunology
70
DOI
出版物ステータス出版済み - 2 1 2016

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Biology

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