Functional analysis of rat renal organic anion transporter OAT-K1: Bidirectional methotrexate transport in apical membrane

Satohiro Masuda, Ayako Takeuchi, Hideyuki Saito, Yukiya Hashimoto, Ken Ichi Inui

研究成果: ジャーナルへの寄稿記事

35 引用 (Scopus)

抄録

Renal organic anion transporter OAT-K1 was stably transfected in MDCK cells and examined for its transport characteristics and membrane localization. OAT-K1 mediated both uptake and efflux of methotrexate in the apical membranes. Immunoblotting showed that the apparent molecular mass of the expressed OAT-K1 was 50 kDa, which was comparable to that found in the rat renal brush-border membranes. The OAT-K1-mediated methotrexate transport was significantly inhibited in the presence of several organic anions such as folate and sulfobromophthalein. These findings suggest that OAT-K1 mediates bidirectional methotrexate transport across the apical membranes, and may be involved in the renal handling of methotrexate. Copyright (C) 1999 Federation of European Biochemical Societies.

元の言語英語
ページ(範囲)128-132
ページ数5
ジャーナルFEBS Letters
459
発行部数1
DOI
出版物ステータス出版済み - 10 1 1999
外部発表Yes

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Organic Anion Transporters
Functional analysis
Methotrexate
Rats
Membranes
Kidney
Sulfobromophthalein
Madin Darby Canine Kidney Cells
Molecular mass
Brushes
Microvilli
Folic Acid
Immunoblotting
Anions

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

これを引用

Functional analysis of rat renal organic anion transporter OAT-K1 : Bidirectional methotrexate transport in apical membrane. / Masuda, Satohiro; Takeuchi, Ayako; Saito, Hideyuki; Hashimoto, Yukiya; Inui, Ken Ichi.

:: FEBS Letters, 巻 459, 番号 1, 01.10.1999, p. 128-132.

研究成果: ジャーナルへの寄稿記事

Masuda, Satohiro ; Takeuchi, Ayako ; Saito, Hideyuki ; Hashimoto, Yukiya ; Inui, Ken Ichi. / Functional analysis of rat renal organic anion transporter OAT-K1 : Bidirectional methotrexate transport in apical membrane. :: FEBS Letters. 1999 ; 巻 459, 番号 1. pp. 128-132.
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T2 - Bidirectional methotrexate transport in apical membrane

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AU - Hashimoto, Yukiya

AU - Inui, Ken Ichi

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