Functional binding of E-selectin to its ligands is enhanced by structural features beyond its lectin domain

Fajr A. Aleisa, Kosuke Sakashita, Jae Man Lee, Dina B. AbuSamra, Bader Al Alwan, Shuho Nozue, Muhammad Tehseen, Samir M. Hamdan, X. Satoshi Habuchi, X. Takahiro Kusakabe, Jasmeen S. Merzaban

研究成果: Contribution to journalArticle査読

5 被引用数 (Scopus)

抄録

Selectins are key to mediating interactions involved in cellular adhesion and migration, underlying processes such as immune responses, metastasis, and transplantation. Selectins are composed of a lectin domain, an epidermal growth factor (EGF)-like domain, multiple short consensus repeats (SCRs), a transmembrane domain, and a cytoplasmic tail. It is well-established that the lectin and EGF domains are required to mediate interactions with ligands; however, the contributions of the other domains in mediating these interactions remain obscure. Using various E-selectin constructs produced in a newly developed silkworm-based expression system and several assays performed under both static and physiological flow conditions, including flow cytometry, glycan array analysis, surface plasmon resonance, and cell-rolling assays, we show here that a reduction in the number of SCR domains is correlated with a decline in functional E-selectin binding to hematopoietic cell E- and/or L-selectin ligand (HCELL) and P-selectin glycoprotein ligand-1 (PSGL-1). Moreover, the binding was significantly improved through E-selectin dimerization and by a substitution (A28H) that mimics an extended conformation of the lectin and EGF domains. Analyses of the association and dissociation rates indicated that the SCR domains, conformational extension, and dimerization collectively contribute to the association rate of E-selectin–ligand binding, whereas just the lectin and EGF domains contribute to the dissociation rate. These findings provide the first evidence of the critical role of the association rate in functional E-selectin–ligand interactions, and they highlight that the SCR domains have an important role that goes beyond the structural extension of the lectin and EGF domains.

本文言語英語
ページ(範囲)3719-3733
ページ数15
ジャーナルJournal of Biological Chemistry
295
11
DOI
出版ステータス出版済み - 3 13 2020

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

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