Functional characterization of an aldose reductase (bmALD1) obtained from the silkworm Bombyx mori

K. Yamamoto, M. Yamaguchi, S. Endo

研究成果: Contribution to journalArticle査読

抄録

We describe a new member of the aldo-keto reductase (AKR) superfamily in the silkworm Bombyx mori. On the basis of its amino acid sequence and phylogenetic tree, this AKR belongs to the AKR1B family and has been designated as bmALD1. In the current study, recombinant bmALD1 was overexpressed, purified to homogeneity and kinetically characterized. We discovered that bmALD1 uses NADPH as a coenzyme to reduce carbonyl compounds such as DL-glyceraldehyde, glucose and 2-nonenal. No NADH-dependent activity was detected. To the best of our knowledge, bmALD1 is only the third AKR characterized in silkworm which, given its substrate specificity, could play a major role in glucose metabolism and antioxidant reactions. Our data provide an increased understanding of insect AKR function.

本文言語英語
ページ(範囲)490-497
ページ数8
ジャーナルInsect Molecular Biology
29
5
DOI
出版ステータス出版済み - 10 1 2020

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Insect Science

フィンガープリント 「Functional characterization of an aldose reductase (bmALD1) obtained from the silkworm Bombyx mori」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル