Functional characterization of blue-light-induced responses and PHOTOTROPIN 1 gene in Welwitschia mirabilis

Kazuhiro Ishishita, Noriyuki Suetsugu, Yuki Hirose, Takeshi Higa, Michio Doi, Masamitsu Wada, Tomonao Matsushita, Eiji Gotoh

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

The blue light (BL) receptor phototropin (phot) is specifically found in green plants; it regulates various BL-induced responses such as phototropism, chloroplast movement, stomatal opening, and leaf flattening. In Arabidopsis thaliana, two phototropins—phot1 and phot2—respond to blue light in overlapping but distinct ways. These BL-receptor-mediated responses enhance the photosynthetic activity of plants under weak light and minimize photodamage under strong light conditions. Welwitschia mirabilis Hook.f. found in the Namib Desert, and it has adapted to severe environmental stresses such as limiting water and strong sunlight. Although the plant has physiologically and ecologically unique features, it is unknown whether phototropin is functional in this plant. In this study, we assessed the functioning of phot-mediated BL responses in W. mirabilis. BL-dependent phototropism and stomatal opening was observed but light-dependent chloroplast movement was not detected. We performed a functional analysis of the PHOT1 gene of W. mirabilis,WmPHOT1, in Arabidopsis thaliana. We generated transgenic A. thaliana lines expressing WmPHOT1 in a phot1 phot2 double mutant background. Several Wmphot1 transgenic plants showed normal growth, although phot1 phot2 double mutant plants showed stunted growth. Furthermore, Wmphot1 transgenic plants showed normal phot1-mediated responses including phototropism, chloroplast accumulation, stomatal opening, and leaf flattening, but lacked the chloroplast avoidance response that is specifically mediated by phot2. Thus, our findings indicate that W. mirabilis possesses typical phot-mediated BL responses that were at least partially mediated by functional phototropin 1, an ortholog of Atphot1.

元の言語英語
ページ(範囲)175-187
ページ数13
ジャーナルJournal of Plant Research
129
発行部数2
DOI
出版物ステータス出版済み - 3 1 2016

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Welwitschia mirabilis
blue light
phototropism
chloroplasts
genes
Arabidopsis thaliana
transgenic plants
stomatal movement
mutants
receptors
leaves
deserts
solar radiation
genetically modified organisms

All Science Journal Classification (ASJC) codes

  • Plant Science

これを引用

Functional characterization of blue-light-induced responses and PHOTOTROPIN 1 gene in Welwitschia mirabilis. / Ishishita, Kazuhiro; Suetsugu, Noriyuki; Hirose, Yuki; Higa, Takeshi; Doi, Michio; Wada, Masamitsu; Matsushita, Tomonao; Gotoh, Eiji.

:: Journal of Plant Research, 巻 129, 番号 2, 01.03.2016, p. 175-187.

研究成果: ジャーナルへの寄稿記事

Ishishita, Kazuhiro ; Suetsugu, Noriyuki ; Hirose, Yuki ; Higa, Takeshi ; Doi, Michio ; Wada, Masamitsu ; Matsushita, Tomonao ; Gotoh, Eiji. / Functional characterization of blue-light-induced responses and PHOTOTROPIN 1 gene in Welwitschia mirabilis. :: Journal of Plant Research. 2016 ; 巻 129, 番号 2. pp. 175-187.
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abstract = "The blue light (BL) receptor phototropin (phot) is specifically found in green plants; it regulates various BL-induced responses such as phototropism, chloroplast movement, stomatal opening, and leaf flattening. In Arabidopsis thaliana, two phototropins—phot1 and phot2—respond to blue light in overlapping but distinct ways. These BL-receptor-mediated responses enhance the photosynthetic activity of plants under weak light and minimize photodamage under strong light conditions. Welwitschia mirabilis Hook.f. found in the Namib Desert, and it has adapted to severe environmental stresses such as limiting water and strong sunlight. Although the plant has physiologically and ecologically unique features, it is unknown whether phototropin is functional in this plant. In this study, we assessed the functioning of phot-mediated BL responses in W. mirabilis. BL-dependent phototropism and stomatal opening was observed but light-dependent chloroplast movement was not detected. We performed a functional analysis of the PHOT1 gene of W. mirabilis,WmPHOT1, in Arabidopsis thaliana. We generated transgenic A. thaliana lines expressing WmPHOT1 in a phot1 phot2 double mutant background. Several Wmphot1 transgenic plants showed normal growth, although phot1 phot2 double mutant plants showed stunted growth. Furthermore, Wmphot1 transgenic plants showed normal phot1-mediated responses including phototropism, chloroplast accumulation, stomatal opening, and leaf flattening, but lacked the chloroplast avoidance response that is specifically mediated by phot2. Thus, our findings indicate that W. mirabilis possesses typical phot-mediated BL responses that were at least partially mediated by functional phototropin 1, an ortholog of Atphot1.",
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AU - Ishishita, Kazuhiro

AU - Suetsugu, Noriyuki

AU - Hirose, Yuki

AU - Higa, Takeshi

AU - Doi, Michio

AU - Wada, Masamitsu

AU - Matsushita, Tomonao

AU - Gotoh, Eiji

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