Functional Conversion of Hemocyanin to Phenoloxidase by Horseshoe Crab Antimicrobial Peptides

Taku Nagai, Tsukasa Osaki, Shun Ichiro Kawabata

研究成果: ジャーナルへの寄稿記事

149 引用 (Scopus)

抄録

Arthropod hemocyanins and phenoloxidases serve different physiological functions as oxygen transporters and enzymes involved in defense reactions, respectively. However, they are equipped with a structurally similar oxygen-binding center. We have shown that the clotting enzyme of the horseshoe crab, Tachypleus tridentatus, functionally converts hemocyanin to phenoloxidase by forming a complex without proteolytic cleavage (Nagai, T., and Kawabata, S. (2000) J. Biol. Chem. 275, 35297-35301). Here we show that chitin-binding antimicrobial peptides of the horseshoe crab induce the intrinsic phenoloxidase activity of hemocyanin. Tachyplesin, a major Tachypleus antimicrobial peptide with an amphiphilic structure, converted the hemocyanin to phenoloxidase. Surface plasmon resonance analysis revealed the specific interaction of tachyplesin with hemocyanin at Kd = 3.4 × 10-6 M. The chemical modification of Trp or Tyr in tachyplesin, but not Lys or Arg, dramatically reduced the affinity to hemocyanin, suggesting that the binding site is located in the hydrophobic face of tachyplesin. Hemocyanin has no affinity with chitin, but it significantly binds to tachyplesin-coated chitin, leading to the expression of phenoloxidase activity. The chitin coated with antimicrobial peptides may serve as a scaffold for the binding of hemocyanin, and the resulting phenoloxidase activity appears to function as a trigger of exoskeleton wound healing.

元の言語英語
ページ(範囲)27166-27170
ページ数5
ジャーナルJournal of Biological Chemistry
276
発行部数29
DOI
出版物ステータス出版済み - 7 20 2001

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Horseshoe Crabs
Hemocyanin
Monophenol Monooxygenase
Peptides
Chitin
Oxygen
Surface Plasmon Resonance
Arthropods
Chemical modification
Surface plasmon resonance
Scaffolds
Wound Healing
Binding Sites
Tachypleus tridentatus tachyplesin peptide
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Functional Conversion of Hemocyanin to Phenoloxidase by Horseshoe Crab Antimicrobial Peptides. / Nagai, Taku; Osaki, Tsukasa; Kawabata, Shun Ichiro.

:: Journal of Biological Chemistry, 巻 276, 番号 29, 20.07.2001, p. 27166-27170.

研究成果: ジャーナルへの寄稿記事

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