Functional effects of expression of hslo Ca2+ activated K+ channels in cultured macrovascular endothelial cells

Masahiro Kamouchi, Dominique Trouet, Christine De Greef, Guy Droogmans, Jan Eggermont, Bernd Nilius

研究成果: Contribution to journalArticle査読

22 被引用数 (Scopus)

抄録

The aim of the present study is to elucidate the effects of the expression of large conductance Ca2+ activated K+ channels (BK(Ca)) in an endothelial cell type normally lacking this channel. The human homologue hslo of BK(Ca) was expressed in cultured bovine pulmonary artery endothelial (CPAE) cells, which have no endogenous BK(Ca). Membrane potential, ionic currents and Ca2+ signals were investigated in non-transfected and transfected cells using a combined patch clamp and Fura-2 fluorescence technique. In non-transfected control CPAE cells, ATP evoked a Ca2+ activated Cl- current (I(Cl,Ca)). The most prominent current component during ATP stimulation in hslo expressing cells was conducted BK(Ca) which resulted in a pronounced transient hyperpolarization. This hyperpolarization, which was absent in non-transfected cells, was enhanced if I(Cl,Ca) was blocked with niflumic acid. The sustained component of the Ca2+ response during ATP stimulation was significantly larger in hslo transfected cells than in non-transfected cells. This plateau level correlated well with the corresponding effects of ATP on the membrane potential, indicating that the expression of cloned BK(Ca) exerts a positive feedback on Ca2+ signals in endothelial cells by counteracting the negative (depolarizing) effect of stimulation of Ca2+-activated Cl- channels.

本文言語英語
ページ(範囲)497-506
ページ数10
ジャーナルCell Calcium
22
6
DOI
出版ステータス出版済み - 12 1997
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生理学
  • 分子生物学
  • 細胞生物学

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