Functional expression of human interleukin-4 receptor α gene on a mouse macrophage cell line

Takeshi Qtsukar, Xoshiaki Nemoto, Kunihiro Yamaqka, Hitoshi Nakasfflma, Hiroaki Niiro, Yosuke Tanaka, Eiichi Qhgaml, Yojiro Arinqbu, Yoshiyuki Niho, Izuhara Kenji

研究成果: ジャーナルへの寄稿記事

抄録

By using B cells of X-linked severe combined immunodeficiency (XSCID) patients, we indicated that mutation of the IL-2 receptor y chain (y c) caused abnormal signal transduction not only of IL-2 but also of IL-4. In this study, we evaluated a functional significance of y c in IL-4 receptor system of monocytes/macrophages. Mouse monocytic cells never responded to human IL-4. We introduced human IL-4 receptor a chain (IL-4Rcc ) gene into a mouse monocytic cell line and established cell lines stably expressing human IL-4Ra. Human IL-4, as well as mouse IL-4, reduced productions of TNF-a, prostaglandins and nitric oxide inducibly produced by such transformed cell lines stimulated with lipopolysaccharide. Neutralizing antibody against y c hardly blocked these functions of human IL-4, indicating that y c may be less important in transducing signals carried out by human IL-4. Further studies are still needed to explain significance of IL-4Rcx and y c of functional IL-4 receptor.

元の言語英語
ページ数1
ジャーナルJapanese Journal of Human Genetics
42
発行部数1
出版物ステータス出版済み - 12 1 1997

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Interleukin-4
Macrophages
Interleukin-4 Receptors
Cell Line
Genes
X-Linked Combined Immunodeficiency Diseases
Transformed Cell Line
Interleukin-2 Receptors
Neutralizing Antibodies
Prostaglandins
Interleukin-2
Lipopolysaccharides
human IL4R protein
Monocytes
Signal Transduction
Nitric Oxide
B-Lymphocytes
Mutation

All Science Journal Classification (ASJC) codes

  • Genetics(clinical)

これを引用

Qtsukar, T., Nemoto, X., Yamaqka, K., Nakasfflma, H., Niiro, H., Tanaka, Y., ... Kenji, I. (1997). Functional expression of human interleukin-4 receptor α gene on a mouse macrophage cell line. Japanese Journal of Human Genetics, 42(1).

Functional expression of human interleukin-4 receptor α gene on a mouse macrophage cell line. / Qtsukar, Takeshi; Nemoto, Xoshiaki; Yamaqka, Kunihiro; Nakasfflma, Hitoshi; Niiro, Hiroaki; Tanaka, Yosuke; Qhgaml, Eiichi; Arinqbu, Yojiro; Niho, Yoshiyuki; Kenji, Izuhara.

:: Japanese Journal of Human Genetics, 巻 42, 番号 1, 01.12.1997.

研究成果: ジャーナルへの寄稿記事

Qtsukar, T, Nemoto, X, Yamaqka, K, Nakasfflma, H, Niiro, H, Tanaka, Y, Qhgaml, E, Arinqbu, Y, Niho, Y & Kenji, I 1997, 'Functional expression of human interleukin-4 receptor α gene on a mouse macrophage cell line', Japanese Journal of Human Genetics, 巻. 42, 番号 1.
Qtsukar, Takeshi ; Nemoto, Xoshiaki ; Yamaqka, Kunihiro ; Nakasfflma, Hitoshi ; Niiro, Hiroaki ; Tanaka, Yosuke ; Qhgaml, Eiichi ; Arinqbu, Yojiro ; Niho, Yoshiyuki ; Kenji, Izuhara. / Functional expression of human interleukin-4 receptor α gene on a mouse macrophage cell line. :: Japanese Journal of Human Genetics. 1997 ; 巻 42, 番号 1.
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AU - Niiro, Hiroaki

AU - Tanaka, Yosuke

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