Functional implication of nucleolin in the mouse first molar development

Ming Xie, Ieyoshi Kobayashi, Tamotsu Kiyoshima, Haruyoshi Yamaza, Jun Ya Honda, Keiko Takahashi, Norio Enoki, Akifumi Akamine, Hidetaka Sakai

研究成果: ジャーナルへの寄稿記事

16 引用 (Scopus)

抄録

We examined the functional implication of nucleolin in the mouse first molar development. Both the nucleolin mRNA and protein expressions were demonstrated in the odontogenic epithelial cells in the early stage and in the inner enamel epithelial layer in the late stage. The expression pattern of nucleolin corresponded to the proliferating cells in the tooth germ, thus showing that nucleolin could possibly be related to cell proliferation. No in situ signal of nucleolin was found in the primary enamel knot (PEK). Furthermore, nucleolin protein was demonstrated in the PEK by immunohistochemistry. The existence of nucleolin protein in the PEK may possibly be related to the apoptosis in the PEK cells. An inhibition assay using the hemagglutinating virus of Japan-liposome containing nucleolin antisense phosphorothioated oligonucleotide (AS S-ODN) in cultured mouse mandibles at embryonic day (E) 11.0 showed a marked growth inhibition of tooth germ. Moreover, no developmental arrest was found in the cultured tooth germ at E15.0 treated with nucleolin AS S-ODN. Real time PCR was performed to examine the mRNA expression of nucleolin-related genes, and a significant reduction in the midkine mRNA expression was thus observed in the mouse mandible after being treated with nucleolin AS S-ODN. This inhibition assay indicated that nucleolin could thus be involved in the early stage of tooth germ initiation and morphogenesis, possibly by regulating the midkine expression.

元の言語英語
ページ(範囲)23275-23283
ページ数9
ジャーナルJournal of Biological Chemistry
282
発行部数32
DOI
出版物ステータス出版済み - 8 10 2007

Fingerprint

Enamels
Tooth Germ
Dental Enamel
Mandible
Messenger RNA
nucleolin
Assays
Sendai virus
Proteins
Antisense Oligonucleotides
Cell proliferation
Morphogenesis
Viruses
Liposomes
Real-Time Polymerase Chain Reaction
Genes
Epithelial Cells
Immunohistochemistry
Cell Proliferation
Apoptosis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Functional implication of nucleolin in the mouse first molar development. / Xie, Ming; Kobayashi, Ieyoshi; Kiyoshima, Tamotsu; Yamaza, Haruyoshi; Honda, Jun Ya; Takahashi, Keiko; Enoki, Norio; Akamine, Akifumi; Sakai, Hidetaka.

:: Journal of Biological Chemistry, 巻 282, 番号 32, 10.08.2007, p. 23275-23283.

研究成果: ジャーナルへの寄稿記事

Xie, M, Kobayashi, I, Kiyoshima, T, Yamaza, H, Honda, JY, Takahashi, K, Enoki, N, Akamine, A & Sakai, H 2007, 'Functional implication of nucleolin in the mouse first molar development', Journal of Biological Chemistry, 巻. 282, 番号 32, pp. 23275-23283. https://doi.org/10.1074/jbc.M610779200
Xie, Ming ; Kobayashi, Ieyoshi ; Kiyoshima, Tamotsu ; Yamaza, Haruyoshi ; Honda, Jun Ya ; Takahashi, Keiko ; Enoki, Norio ; Akamine, Akifumi ; Sakai, Hidetaka. / Functional implication of nucleolin in the mouse first molar development. :: Journal of Biological Chemistry. 2007 ; 巻 282, 番号 32. pp. 23275-23283.
@article{a3cc4f418dfc4cd59cc2001d559c9a24,
title = "Functional implication of nucleolin in the mouse first molar development",
abstract = "We examined the functional implication of nucleolin in the mouse first molar development. Both the nucleolin mRNA and protein expressions were demonstrated in the odontogenic epithelial cells in the early stage and in the inner enamel epithelial layer in the late stage. The expression pattern of nucleolin corresponded to the proliferating cells in the tooth germ, thus showing that nucleolin could possibly be related to cell proliferation. No in situ signal of nucleolin was found in the primary enamel knot (PEK). Furthermore, nucleolin protein was demonstrated in the PEK by immunohistochemistry. The existence of nucleolin protein in the PEK may possibly be related to the apoptosis in the PEK cells. An inhibition assay using the hemagglutinating virus of Japan-liposome containing nucleolin antisense phosphorothioated oligonucleotide (AS S-ODN) in cultured mouse mandibles at embryonic day (E) 11.0 showed a marked growth inhibition of tooth germ. Moreover, no developmental arrest was found in the cultured tooth germ at E15.0 treated with nucleolin AS S-ODN. Real time PCR was performed to examine the mRNA expression of nucleolin-related genes, and a significant reduction in the midkine mRNA expression was thus observed in the mouse mandible after being treated with nucleolin AS S-ODN. This inhibition assay indicated that nucleolin could thus be involved in the early stage of tooth germ initiation and morphogenesis, possibly by regulating the midkine expression.",
author = "Ming Xie and Ieyoshi Kobayashi and Tamotsu Kiyoshima and Haruyoshi Yamaza and Honda, {Jun Ya} and Keiko Takahashi and Norio Enoki and Akifumi Akamine and Hidetaka Sakai",
year = "2007",
month = "8",
day = "10",
doi = "10.1074/jbc.M610779200",
language = "English",
volume = "282",
pages = "23275--23283",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "32",

}

TY - JOUR

T1 - Functional implication of nucleolin in the mouse first molar development

AU - Xie, Ming

AU - Kobayashi, Ieyoshi

AU - Kiyoshima, Tamotsu

AU - Yamaza, Haruyoshi

AU - Honda, Jun Ya

AU - Takahashi, Keiko

AU - Enoki, Norio

AU - Akamine, Akifumi

AU - Sakai, Hidetaka

PY - 2007/8/10

Y1 - 2007/8/10

N2 - We examined the functional implication of nucleolin in the mouse first molar development. Both the nucleolin mRNA and protein expressions were demonstrated in the odontogenic epithelial cells in the early stage and in the inner enamel epithelial layer in the late stage. The expression pattern of nucleolin corresponded to the proliferating cells in the tooth germ, thus showing that nucleolin could possibly be related to cell proliferation. No in situ signal of nucleolin was found in the primary enamel knot (PEK). Furthermore, nucleolin protein was demonstrated in the PEK by immunohistochemistry. The existence of nucleolin protein in the PEK may possibly be related to the apoptosis in the PEK cells. An inhibition assay using the hemagglutinating virus of Japan-liposome containing nucleolin antisense phosphorothioated oligonucleotide (AS S-ODN) in cultured mouse mandibles at embryonic day (E) 11.0 showed a marked growth inhibition of tooth germ. Moreover, no developmental arrest was found in the cultured tooth germ at E15.0 treated with nucleolin AS S-ODN. Real time PCR was performed to examine the mRNA expression of nucleolin-related genes, and a significant reduction in the midkine mRNA expression was thus observed in the mouse mandible after being treated with nucleolin AS S-ODN. This inhibition assay indicated that nucleolin could thus be involved in the early stage of tooth germ initiation and morphogenesis, possibly by regulating the midkine expression.

AB - We examined the functional implication of nucleolin in the mouse first molar development. Both the nucleolin mRNA and protein expressions were demonstrated in the odontogenic epithelial cells in the early stage and in the inner enamel epithelial layer in the late stage. The expression pattern of nucleolin corresponded to the proliferating cells in the tooth germ, thus showing that nucleolin could possibly be related to cell proliferation. No in situ signal of nucleolin was found in the primary enamel knot (PEK). Furthermore, nucleolin protein was demonstrated in the PEK by immunohistochemistry. The existence of nucleolin protein in the PEK may possibly be related to the apoptosis in the PEK cells. An inhibition assay using the hemagglutinating virus of Japan-liposome containing nucleolin antisense phosphorothioated oligonucleotide (AS S-ODN) in cultured mouse mandibles at embryonic day (E) 11.0 showed a marked growth inhibition of tooth germ. Moreover, no developmental arrest was found in the cultured tooth germ at E15.0 treated with nucleolin AS S-ODN. Real time PCR was performed to examine the mRNA expression of nucleolin-related genes, and a significant reduction in the midkine mRNA expression was thus observed in the mouse mandible after being treated with nucleolin AS S-ODN. This inhibition assay indicated that nucleolin could thus be involved in the early stage of tooth germ initiation and morphogenesis, possibly by regulating the midkine expression.

UR - http://www.scopus.com/inward/record.url?scp=34548167304&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548167304&partnerID=8YFLogxK

U2 - 10.1074/jbc.M610779200

DO - 10.1074/jbc.M610779200

M3 - Article

C2 - 17562718

AN - SCOPUS:34548167304

VL - 282

SP - 23275

EP - 23283

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 32

ER -