Functional requirement of CCN2 for intramembranous bone formation in embryonic mice

Harumi Kawaki, Satoshi Kubota, Akiko Suzuki, Tomohiro Yamada, Tatsushi Matsumura, Toshiko Mandai, Mayumi Yao, Takeyasu Maeda, Karen M. Lyons, Masaharu Takigawa

研究成果: ジャーナルへの寄稿記事

40 引用 (Scopus)

抄録

CCN2 is best known as a promoter of chondrocyte differentiation among the CCN family members, and Ccn2 null mutant mice display skeletal dysmorphisms. However, little is known concerning the roles of CCN2 during bone formation. We herein present a comparative analysis of wild-type and Ccn2 null mice to investigate the roles of CCN2 in bone development. Multiple histochemical methods were employed to analyze the effects of CCN2 deletion in vivo, and effects of CCN2 on the osteogenic response were evaluated with the isolated and cultured osteoblasts. As a result, we found a drastic reduction of the osteoblastic phenotype in Ccn2 null mutants. Importantly, addition of exogenous CCN2 promoted every step of osteoblast differentiation and rescued the attenuated activities of the Ccn2 null osteoblasts. These results suggest that CCN2 is required not only for the regulation of cartilage and subsequent events, but also for the normal intramembranous bone development.

元の言語英語
ページ(範囲)450-456
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
366
発行部数2
DOI
出版物ステータス出版済み - 2 8 2008
外部発表Yes

Fingerprint

Osteoblasts
Osteogenesis
Bone
Bone Development
Cartilage
Chondrocytes
Phenotype

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Functional requirement of CCN2 for intramembranous bone formation in embryonic mice. / Kawaki, Harumi; Kubota, Satoshi; Suzuki, Akiko; Yamada, Tomohiro; Matsumura, Tatsushi; Mandai, Toshiko; Yao, Mayumi; Maeda, Takeyasu; Lyons, Karen M.; Takigawa, Masaharu.

:: Biochemical and Biophysical Research Communications, 巻 366, 番号 2, 08.02.2008, p. 450-456.

研究成果: ジャーナルへの寄稿記事

Kawaki, H, Kubota, S, Suzuki, A, Yamada, T, Matsumura, T, Mandai, T, Yao, M, Maeda, T, Lyons, KM & Takigawa, M 2008, 'Functional requirement of CCN2 for intramembranous bone formation in embryonic mice', Biochemical and Biophysical Research Communications, 巻. 366, 番号 2, pp. 450-456. https://doi.org/10.1016/j.bbrc.2007.11.155
Kawaki, Harumi ; Kubota, Satoshi ; Suzuki, Akiko ; Yamada, Tomohiro ; Matsumura, Tatsushi ; Mandai, Toshiko ; Yao, Mayumi ; Maeda, Takeyasu ; Lyons, Karen M. ; Takigawa, Masaharu. / Functional requirement of CCN2 for intramembranous bone formation in embryonic mice. :: Biochemical and Biophysical Research Communications. 2008 ; 巻 366, 番号 2. pp. 450-456.
@article{557ae0b07be247e7aa409c4497f2acd7,
title = "Functional requirement of CCN2 for intramembranous bone formation in embryonic mice",
abstract = "CCN2 is best known as a promoter of chondrocyte differentiation among the CCN family members, and Ccn2 null mutant mice display skeletal dysmorphisms. However, little is known concerning the roles of CCN2 during bone formation. We herein present a comparative analysis of wild-type and Ccn2 null mice to investigate the roles of CCN2 in bone development. Multiple histochemical methods were employed to analyze the effects of CCN2 deletion in vivo, and effects of CCN2 on the osteogenic response were evaluated with the isolated and cultured osteoblasts. As a result, we found a drastic reduction of the osteoblastic phenotype in Ccn2 null mutants. Importantly, addition of exogenous CCN2 promoted every step of osteoblast differentiation and rescued the attenuated activities of the Ccn2 null osteoblasts. These results suggest that CCN2 is required not only for the regulation of cartilage and subsequent events, but also for the normal intramembranous bone development.",
author = "Harumi Kawaki and Satoshi Kubota and Akiko Suzuki and Tomohiro Yamada and Tatsushi Matsumura and Toshiko Mandai and Mayumi Yao and Takeyasu Maeda and Lyons, {Karen M.} and Masaharu Takigawa",
year = "2008",
month = "2",
day = "8",
doi = "10.1016/j.bbrc.2007.11.155",
language = "English",
volume = "366",
pages = "450--456",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Functional requirement of CCN2 for intramembranous bone formation in embryonic mice

AU - Kawaki, Harumi

AU - Kubota, Satoshi

AU - Suzuki, Akiko

AU - Yamada, Tomohiro

AU - Matsumura, Tatsushi

AU - Mandai, Toshiko

AU - Yao, Mayumi

AU - Maeda, Takeyasu

AU - Lyons, Karen M.

AU - Takigawa, Masaharu

PY - 2008/2/8

Y1 - 2008/2/8

N2 - CCN2 is best known as a promoter of chondrocyte differentiation among the CCN family members, and Ccn2 null mutant mice display skeletal dysmorphisms. However, little is known concerning the roles of CCN2 during bone formation. We herein present a comparative analysis of wild-type and Ccn2 null mice to investigate the roles of CCN2 in bone development. Multiple histochemical methods were employed to analyze the effects of CCN2 deletion in vivo, and effects of CCN2 on the osteogenic response were evaluated with the isolated and cultured osteoblasts. As a result, we found a drastic reduction of the osteoblastic phenotype in Ccn2 null mutants. Importantly, addition of exogenous CCN2 promoted every step of osteoblast differentiation and rescued the attenuated activities of the Ccn2 null osteoblasts. These results suggest that CCN2 is required not only for the regulation of cartilage and subsequent events, but also for the normal intramembranous bone development.

AB - CCN2 is best known as a promoter of chondrocyte differentiation among the CCN family members, and Ccn2 null mutant mice display skeletal dysmorphisms. However, little is known concerning the roles of CCN2 during bone formation. We herein present a comparative analysis of wild-type and Ccn2 null mice to investigate the roles of CCN2 in bone development. Multiple histochemical methods were employed to analyze the effects of CCN2 deletion in vivo, and effects of CCN2 on the osteogenic response were evaluated with the isolated and cultured osteoblasts. As a result, we found a drastic reduction of the osteoblastic phenotype in Ccn2 null mutants. Importantly, addition of exogenous CCN2 promoted every step of osteoblast differentiation and rescued the attenuated activities of the Ccn2 null osteoblasts. These results suggest that CCN2 is required not only for the regulation of cartilage and subsequent events, but also for the normal intramembranous bone development.

UR - http://www.scopus.com/inward/record.url?scp=37449000249&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=37449000249&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2007.11.155

DO - 10.1016/j.bbrc.2007.11.155

M3 - Article

VL - 366

SP - 450

EP - 456

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -