Functional significance of octameric RuvA for a branch migration complex from Thermus thermophilus

Yoshie Fujiwara, Kouta Mayanagi, Kosuke Morikawa

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

The RuvAB complex promotes migration of Holliday junction at the late stage of homologous recombination. The RuvA tetramer specifically recognizes Holliday junction to form two types of complexes. A single tetramer is bound to the open configuration of the junction DNA in complex I, while the octameric RuvA core structure sandwiches the same junction in complex II. The hexameric RuvB rings, symmetrically bound to both sides of RuvA on Holliday junction, pump out DNA duplexes, depending upon ATP hydrolysis. We investigated functional differences between the wild-type RuvA from Thermus thermophilus and mutants impaired the ability of complex II formation. These mutant RuvA, exclusively forming complex I, reduced activities of branch migration and ATP hydrolysis, suggesting that the octameric RuvA is essential for efficient branch migration. Together with our recent electron microscopic analysis, this finding provides important insights into functional roles of complex II in the coordinated branch migration mechanism.

元の言語英語
ページ(範囲)426-431
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
366
発行部数2
DOI
出版物ステータス出版済み - 2 8 2008
外部発表Yes

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Cruciform DNA
Thermus thermophilus
Hydrolysis
Adenosine Triphosphate
Sandwich structures
DNA
Homologous Recombination
Pumps
Electrons

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Functional significance of octameric RuvA for a branch migration complex from Thermus thermophilus. / Fujiwara, Yoshie; Mayanagi, Kouta; Morikawa, Kosuke.

:: Biochemical and Biophysical Research Communications, 巻 366, 番号 2, 08.02.2008, p. 426-431.

研究成果: ジャーナルへの寄稿記事

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