Functions of multiple clamp and clamp-loader complexes in eukaryotic DNA replication

研究成果: 著書/レポートタイプへの貢献

3 引用 (Scopus)

抄録

Proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) were identified in the late 1980s as essential factors for replication of simian virus 40 DNA in human cells, by reconstitution of the reaction in vitro. Initially, they were only thought to be involved in the elongation stage of DNA replication. Subsequent studies have demonstrated that PCNA functions as more than a replication factor, through its involvement in multiple protein-protein interactions. PCNA appears as a functional hub on replicating and replicated chromosomal DNA and has an essential role in the maintenance genome integrity in proliferating cells. Eukaryotes have multiple paralogues of sliding clamp, PCNA and its loader, RFC. The PCNA paralogues, RAD9, HUS1, and RAD1 form the heterotrimeric 9-1-1 ring that is similar to the PCNA homotrimeric ring, and the 9-1-1 clamp complex is loaded onto sites of DNA damage by its specific loader RAD17-RFC. This alternative clamp-loader system transmits DNA-damage signals in genomic DNA to the checkpoint-activation network and the DNA-repair apparatus. Another two alternative loader complexes, CTF18-RFC and ELG1-RFC, have roles that are distinguishable from the role of the canonical loader, RFC. CTF18-RFC interacts with one of the replicative DNA polymerases, Polε, and loads PCNA onto leading-strand DNA, and ELG1-RFC unloads PCNA after ligation of lagging-strand DNA. In the progression of S phase, these alternative PCNA loaders maintain appropriate amounts of PCNA on the replicating sister DNAs to ensure that specific enzymes are tethered at specific chromosomal locations.

元の言語英語
ホスト出版物のタイトルAdvances in Experimental Medicine and Biology
出版者Springer New York LLC
ページ135-162
ページ数28
DOI
出版物ステータス出版済み - 1 1 2017

出版物シリーズ

名前Advances in Experimental Medicine and Biology
1042
ISSN(印刷物)0065-2598
ISSN(電子版)2214-8019

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Replication Protein C
Loaders
Clamping devices
Proliferating Cell Nuclear Antigen
DNA Replication
DNA
DNA Damage
Simian virus 40
DNA-Directed DNA Polymerase
Eukaryota
S Phase
Viruses
DNA Repair
Ligation
Elongation
Proteins
Repair
Genes
Chemical activation
Cells

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Ohashi, E., & Tsurimoto, T. (2017). Functions of multiple clamp and clamp-loader complexes in eukaryotic DNA replication. : Advances in Experimental Medicine and Biology (pp. 135-162). (Advances in Experimental Medicine and Biology; 巻数 1042). Springer New York LLC. https://doi.org/10.1007/978-981-10-6955-0_7

Functions of multiple clamp and clamp-loader complexes in eukaryotic DNA replication. / Ohashi, Eiji; Tsurimoto, Toshiki.

Advances in Experimental Medicine and Biology. Springer New York LLC, 2017. p. 135-162 (Advances in Experimental Medicine and Biology; 巻 1042).

研究成果: 著書/レポートタイプへの貢献

Ohashi, E & Tsurimoto, T 2017, Functions of multiple clamp and clamp-loader complexes in eukaryotic DNA replication. : Advances in Experimental Medicine and Biology. Advances in Experimental Medicine and Biology, 巻. 1042, Springer New York LLC, pp. 135-162. https://doi.org/10.1007/978-981-10-6955-0_7
Ohashi E, Tsurimoto T. Functions of multiple clamp and clamp-loader complexes in eukaryotic DNA replication. : Advances in Experimental Medicine and Biology. Springer New York LLC. 2017. p. 135-162. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-981-10-6955-0_7
Ohashi, Eiji ; Tsurimoto, Toshiki. / Functions of multiple clamp and clamp-loader complexes in eukaryotic DNA replication. Advances in Experimental Medicine and Biology. Springer New York LLC, 2017. pp. 135-162 (Advances in Experimental Medicine and Biology).
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