Galactinol synthase gene of Coptis japonica is involved in berberine tolerance

Kojiro Takanashi, Nobukazu Shitan, Akifumi Sugiyama, Yoshihisa Kamimoto, Masafumi Hamamoto, Tomoko Iwaki, Kaoru Takegawa, Kazufumi Yazaki

研究成果: ジャーナルへの寄稿学術誌査読

10 被引用数 (Scopus)

抄録

Many plant secondary metabolites show strong biological activities and are potentially also toxic to plants, while plants producing such active compounds are usually insensitive to their own metabolites, suggesting that they have species-specific detoxification mechanisms. In order to clarify the detoxification mechanism of alkaloids, we used cultured cells of Coptis japonica, which are capable of producing a yellow benzylisoquinoline alkaloid, berberine, and accumulate it in the vacuole. Unlike other plant cells that do not produce berberine, C. japonica shows strong tolerance to this alkaloid. We established a fission yeast strain that was sensitive to berberine and performed functional screening using a C. japonica cDNA library. One cDNA clone, which conferred clear berberine tolerance, encoded galactinol synthase (CjGolS). The possible role of CjGolS in berberine tolerance is discussed.

本文言語英語
ページ(範囲)398-405
ページ数8
ジャーナルBioscience, Biotechnology and Biochemistry
72
2
DOI
出版ステータス出版済み - 2008
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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