Galactofuranosidase from JHA 19 Streptomyces sp.

subcloning and biochemical characterization

Mateja Seničar, Laurent Legentil, Vincent Ferrières, Svetlana V. Eliseeva, Stéphane Petoud, Kaoru Takegawa, Pierre Lafite, Richard Daniellou

研究成果: ジャーナルへの寄稿記事

抄録

Despite the crucial role of the rare galactofuranose (Galf) in many pathogenic micro-organisms and our increased knowledge of its metabolism, there is still a lack of recombinant and efficient galactofuranoside hydrolase available for chemo-enzymatic synthetic purposes of specific galactofuranosyl-conjugates. Subcloning of the Galf-ase from JHA 19 Streptomyces sp. and its further overexpression lead us to the production of this enzyme with a yield of 0.5 mg/L of culture. It exhibits substrate specificity exclusively towards pNP β-D-Galf, giving a KM value of 250 μM, and the highest enzymatic efficiency ever observed of 14 mM−1 s−1. It proved to be stable to temperature up to 60 °C and to at least 4 freeze-thaw's cycles. Thus, Galf-ase demonstrated to be an efficient and stable biocatalyst with greatly improved specificity toward the galactofuranosyl entity, thus paving the way to the further development of transglycosylation and thioligation reactions.

元の言語英語
ページ(範囲)35-41
ページ数7
ジャーナルCarbohydrate Research
480
DOI
出版物ステータス出版済み - 7 1 2019

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Streptomyces
Hydrolases
Enzymes
Substrate Specificity
Metabolism
Temperature
Substrates

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

これを引用

Seničar, M., Legentil, L., Ferrières, V., Eliseeva, S. V., Petoud, S., Takegawa, K., ... Daniellou, R. (2019). Galactofuranosidase from JHA 19 Streptomyces sp. subcloning and biochemical characterization. Carbohydrate Research, 480, 35-41. https://doi.org/10.1016/j.carres.2019.05.011

Galactofuranosidase from JHA 19 Streptomyces sp. subcloning and biochemical characterization. / Seničar, Mateja; Legentil, Laurent; Ferrières, Vincent; Eliseeva, Svetlana V.; Petoud, Stéphane; Takegawa, Kaoru; Lafite, Pierre; Daniellou, Richard.

:: Carbohydrate Research, 巻 480, 01.07.2019, p. 35-41.

研究成果: ジャーナルへの寄稿記事

Seničar, M, Legentil, L, Ferrières, V, Eliseeva, SV, Petoud, S, Takegawa, K, Lafite, P & Daniellou, R 2019, 'Galactofuranosidase from JHA 19 Streptomyces sp. subcloning and biochemical characterization', Carbohydrate Research, 巻. 480, pp. 35-41. https://doi.org/10.1016/j.carres.2019.05.011
Seničar, Mateja ; Legentil, Laurent ; Ferrières, Vincent ; Eliseeva, Svetlana V. ; Petoud, Stéphane ; Takegawa, Kaoru ; Lafite, Pierre ; Daniellou, Richard. / Galactofuranosidase from JHA 19 Streptomyces sp. subcloning and biochemical characterization. :: Carbohydrate Research. 2019 ; 巻 480. pp. 35-41.
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AU - Petoud, Stéphane

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AU - Daniellou, Richard

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