By means of 5α-reductase inhibitory activity-guided fractionation, a new lanostanoid, 15α-hydroxy-3-oxolanosta- 7, 9(11), 24(E)-trien-26-oic acid, named as ganoderic acid TR, was isolated from the ethanol extract of the fruiting bodies of Ganoderma lucidum (Fr.) Karst (Ganodermataceae). The compound showed potent inhibitory activity with an IC50 of 8.5 μM. Modified Mosher's method established the S-configuration of the secondary hydroxyl group at C-15. In addition, a carboxyl group on the side chain of ganoderic acid TR is essential to elicit the inhibitory activity because of the considerably lower activity of its methyl ester.
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