GC-MS-based metabolic signatures reveal comparative steroidogenic pathways between fetal and adult mouse testes

Soyun Han, Takashi Baba, Shogo Yanai, Dong Jun Byun, Ken ichirou Morohashi, Jae Hong Kim, Man Ho Choi

研究成果: ジャーナルへの寄稿学術誌査読

1 被引用数 (Scopus)


Background: Previous studies on gonadal steroidogenesis have not compared metabolic pathways between fetal and adult mouse testes to date. Objectives: To evaluate comparative metabolic signatures of testicular steroids between fetus and adult mice using gas chromatography-mass spectrometry (GC-MS)-based steroid profiling. Materials and methods: GC-MS with molecular-specific scan modes was optimized for selective and sensitive detection of 23 androgens, 7 estrogens, 14 progestogens, and 13 corticoids from mouse testes with a quantification limit of 0.1-5.0 ng/mL and reproducibility (coefficient of variation: 0.3%-19.9%). Based on 26 steroids quantitatively detected in testes, comparative steroid signatures were analyzed for mouse testes of 8 fetuses on embryonic day 16.5 and 8 adults on postnatal days 56-60. Results: In contrast to large amounts of steroids in adult testes (P <.0002), all testicular levels per weight unit of protein were significantly increased in fetal testes (P <.002, except 6β-hydroxytestosterone of P =.065). Both 11β-hydroxyandrostenedione and 7α-hydroxytestosterone were only measurable in fetal testes, and metabolic ratios of testosterone to androstenediol and androstenedione were also increased in fetal testes (P <.05 for both). Discussion and conclusion: Testicular steroid signatures showed that both steroidogenic Δ4 and Δ5 pathways in the production of testosterone were activated more during prenatal development. Both 7α- and 11β-hydroxylations were predominant, while hydroxylations at C-6, C-15, and C-16 of testosterone and androstenedione were decreased in the fetus. The present GC-MS-based steroid profiling may facilitate understanding of the development of testicular steroidogenesis.

出版ステータス出版済み - 1月 2021

All Science Journal Classification (ASJC) codes

  • 内分泌学、糖尿病および代謝内科学
  • 生殖医学
  • 内分泌学
  • 泌尿器学


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