Gene cluster responsible for secretion of and immunity to multiple bacteriocins, the NKR-5-3 enterocins

Naoki Ishibashi, Kohei Himeno, Yoshimitsu Masuda, Rodney Honrada Perez, Shun Iwatani, Takeshi Zendo, Pongtep Wilaipun, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

Enterococcus faecium NKR-5-3, isolated from Thai fermented fish, is characterized by the unique ability to produce five bacteriocins, namely, enterocins NKR-5-3A, -B, -C, -D, and -Z (Ent53A, Ent53B, Ent53C, Ent53D, and Ent53Z). Genetic analysis with a genome library revealed that the bacteriocin structural genes (enkA [ent53A], enkC [ent53C], enkD [ent53D], and enkZ [ent53Z]) that encode these peptides (except for Ent53B) are located in close proximity to each other. This NKR-5-3ACDZ (Ent53ACDZ) enterocin gene cluster (approximately 13 kb long) includes certain bacteriocin biosynthetic genes such as an ABC transporter gene (enkT), two immunity genes (enkIaz and enkIc), a response regulator (enkR), and a histidine protein kinase (enkK). Heterologous- expression studies of enkT and ΔenkT mutant strains showed that enkT is responsible for the secretion of Ent53A, Ent53C, Ent53D, and Ent53Z, suggesting that EnkT is a wide-range ABC transporter that contributes to the effective production of these bacteriocins. In addition, EnkIaz and EnkIc were found to confer self-immunity to the respective bacteriocins. Furthermore, bacteriocin induction assays performed with the ΔenkRK mutant strain showed that EnkR and EnkK are regulatory proteins responsible for bacteriocin production and that, together with Ent53D, they constitute a three-component regulatory system. Thus, the Ent53ACDZ gene cluster is essential for the biosynthesis and regulation of NKR-5-3 enterocins, and this is, to our knowledge, the first report that demonstrates the secretion of multiple bacteriocins by an ABC transporter.

元の言語英語
ページ(範囲)6647-6655
ページ数9
ジャーナルApplied and Environmental Microbiology
80
発行部数21
DOI
出版物ステータス出版済み - 1 1 2014

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enterocins
Bacteriocins
bacteriocins
Multigene Family
immunity
multigene family
secretion
Immunity
gene
ABC transporters
ATP-Binding Cassette Transporters
Genes
protein-histidine kinases
protein
genetic analysis
fermented fish
Enterococcus faecium
peptide
mutants
genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

これを引用

Gene cluster responsible for secretion of and immunity to multiple bacteriocins, the NKR-5-3 enterocins. / Ishibashi, Naoki; Himeno, Kohei; Masuda, Yoshimitsu; Perez, Rodney Honrada; Iwatani, Shun; Zendo, Takeshi; Wilaipun, Pongtep; Leelawatcharamas, Vichien; Nakayama, Jiro; Sonomoto, Kenji.

:: Applied and Environmental Microbiology, 巻 80, 番号 21, 01.01.2014, p. 6647-6655.

研究成果: ジャーナルへの寄稿記事

Ishibashi, N, Himeno, K, Masuda, Y, Perez, RH, Iwatani, S, Zendo, T, Wilaipun, P, Leelawatcharamas, V, Nakayama, J & Sonomoto, K 2014, 'Gene cluster responsible for secretion of and immunity to multiple bacteriocins, the NKR-5-3 enterocins', Applied and Environmental Microbiology, 巻. 80, 番号 21, pp. 6647-6655. https://doi.org/10.1128/AEM.02312-14
Ishibashi, Naoki ; Himeno, Kohei ; Masuda, Yoshimitsu ; Perez, Rodney Honrada ; Iwatani, Shun ; Zendo, Takeshi ; Wilaipun, Pongtep ; Leelawatcharamas, Vichien ; Nakayama, Jiro ; Sonomoto, Kenji. / Gene cluster responsible for secretion of and immunity to multiple bacteriocins, the NKR-5-3 enterocins. :: Applied and Environmental Microbiology. 2014 ; 巻 80, 番号 21. pp. 6647-6655.
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title = "Gene cluster responsible for secretion of and immunity to multiple bacteriocins, the NKR-5-3 enterocins",
abstract = "Enterococcus faecium NKR-5-3, isolated from Thai fermented fish, is characterized by the unique ability to produce five bacteriocins, namely, enterocins NKR-5-3A, -B, -C, -D, and -Z (Ent53A, Ent53B, Ent53C, Ent53D, and Ent53Z). Genetic analysis with a genome library revealed that the bacteriocin structural genes (enkA [ent53A], enkC [ent53C], enkD [ent53D], and enkZ [ent53Z]) that encode these peptides (except for Ent53B) are located in close proximity to each other. This NKR-5-3ACDZ (Ent53ACDZ) enterocin gene cluster (approximately 13 kb long) includes certain bacteriocin biosynthetic genes such as an ABC transporter gene (enkT), two immunity genes (enkIaz and enkIc), a response regulator (enkR), and a histidine protein kinase (enkK). Heterologous- expression studies of enkT and ΔenkT mutant strains showed that enkT is responsible for the secretion of Ent53A, Ent53C, Ent53D, and Ent53Z, suggesting that EnkT is a wide-range ABC transporter that contributes to the effective production of these bacteriocins. In addition, EnkIaz and EnkIc were found to confer self-immunity to the respective bacteriocins. Furthermore, bacteriocin induction assays performed with the ΔenkRK mutant strain showed that EnkR and EnkK are regulatory proteins responsible for bacteriocin production and that, together with Ent53D, they constitute a three-component regulatory system. Thus, the Ent53ACDZ gene cluster is essential for the biosynthesis and regulation of NKR-5-3 enterocins, and this is, to our knowledge, the first report that demonstrates the secretion of multiple bacteriocins by an ABC transporter.",
author = "Naoki Ishibashi and Kohei Himeno and Yoshimitsu Masuda and Perez, {Rodney Honrada} and Shun Iwatani and Takeshi Zendo and Pongtep Wilaipun and Vichien Leelawatcharamas and Jiro Nakayama and Kenji Sonomoto",
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AU - Ishibashi, Naoki

AU - Himeno, Kohei

AU - Masuda, Yoshimitsu

AU - Perez, Rodney Honrada

AU - Iwatani, Shun

AU - Zendo, Takeshi

AU - Wilaipun, Pongtep

AU - Leelawatcharamas, Vichien

AU - Nakayama, Jiro

AU - Sonomoto, Kenji

PY - 2014/1/1

Y1 - 2014/1/1

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