Gene encoding a replication initiator protein and replication origin of conjugative plasmid pSA1.1 of Streptomyces cyaneus ATCC 14921

Eiji Yokoyama, Yumi Matsuzaki, Katsumi Doi, Seiya Ogata

研究成果: ジャーナルへの寄稿学術誌査読

7 被引用数 (Scopus)

抄録

pSA1.1 is a 9.1-kb multicopy plasmid originally isolated from Streptomyces cyaneus (formerly S. azureus) ATCC 14921. This plasmid accumulates single-stranded DNA in S. lividans and is therefore considered to replicate by a rolling-circle replication. In the present work, the rep gene encoding the replication initiator protein and the replication origin ori of pSA1.1 were determined. The rep and ori are located on separate regions. The Rep protein of pSA1.1 belongs to superfamily I which includes A proteins of phages. Nucleotide sequence of the surrounding putative nicking site of pSA1.1 shows good agreement with those of the pC194 group plasmids and phages. The direction of replication was also determined. Copyright (C) 1998 Federation of European Microbiological Societies.

本文言語英語
ページ(範囲)103-109
ページ数7
ジャーナルFEMS microbiology letters
169
1
DOI
出版ステータス出版済み - 12月 1 1998

!!!All Science Journal Classification (ASJC) codes

  • 微生物学
  • 分子生物学
  • 遺伝学

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