Gene expression analysis of CCN protein in bone under mechanical stress

Teruko Takano-Yamamoto, Tomohiro Fukunaga, Nobuo Takeshita

研究成果: Chapter in Book/Report/Conference proceedingChapter

4 被引用数 (Scopus)

抄録

To investigate mechanical-dependent bone remodeling, we had previously applied various types of mechanical loading onto the teeth of rats and mice. In vitro cultured bone cells were then used to elucidate the mechanisms underlying the specifi c phenomenon revealed by in vivo experiments. This review describes the techniques used to upregulate CCN2 expression in bone cells produced by different types of mechanical stress, such as fl uid shear stress and substrate strain in vitro, and compression or tension force in vivo.

本文言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ283-308
ページ数26
DOI
出版ステータス出版済み - 2017
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
1489
ISSN(印刷版)1064-3745

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

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