Gene Expression in on-Chip Membrane-Bound Artificial Cells

Ziane Izri, David Garenne, Vincent Noireaux, Yusuke T. Maeda

研究成果: ジャーナルへの寄稿学術誌査読

17 被引用数 (Scopus)

抄録

Artificial cells made of molecular components and lipid membrane are emerging platforms to characterize living systems properties. Cell-free transcription-translation (TXTL) offers advantages for the bottom-up synthesis of cellular reactors. Yet, scaling up their design within well-defined geometries remains challenging. We present a microfluidic device hosting TXTL reactions of a reporter gene in thousands of microwells separated from an external buffer by a phospholipid membrane. In the presence of nutrients in the buffer, microreactors are stable beyond 24 h and yield a few mg/mL of proteins. Nutrients in the external solution feed the TXTL reaction at the picoliter scale via passive transport across the phospholipid membrane of each microfluidic well, despite the absence of pores. Replacing nutrients with an inert polymer and fatty acids at an isotonic concentration reduces microreactors efficiency, and a significant fraction yields no protein. This emphasizes the crucial role of the membrane for designing cell-free TXTL microreactors as efficient artificial cells.

本文言語英語
ページ(範囲)1705-1712
ページ数8
ジャーナルACS Synthetic Biology
8
8
DOI
出版ステータス出版済み - 8月 16 2019

!!!All Science Journal Classification (ASJC) codes

  • 生体医工学
  • 生化学、遺伝学、分子生物学(その他)

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