Gene structure and multiple mRNA species of Drosophila melanogaster aldolase generating three isozymes with different enzymatic properties

Tatsuo Kai, Yasushi Sugimoto, Takahiro Kusakabe, Rong Zhang, Katsumi Koga, Katsuji Hori

研究成果: Contribution to journalArticle査読

16 被引用数 (Scopus)

抄録

Genomic clones encoding the Drosophila aldolase gene were isolated and the organization of the gene was determined. The protein-coding region spanning nearly 3.5 kb consists of five coding exons (exon 2, 3, 4α4β, and Aγ). The insect exon 2 corresponds to exons 2 to 7 of vertebrate aldolase genes and thus appears to have been formed by the fusion of these 6 exons into a single exon daring evolution. The Drosophila aldolase gene is predicted to generate mRNAs for three isozymes (α-, β-, and γ-types) from the primary transcripts by alternative usage of the final three exons. The reverse transcriptase-PCR assay revealed the occurrence of mRNAs for the three isozymic forms at different developmental stages, and tissue-specific expression was also found to occur in adult flies. In addition to the usual type mRNA species for the α-, β-, and γ-isozymes, two novel forms of mRNAs, αbeta;- and βγ-type mRNAs, were detected tissue-specifically in adult files, although their functions are unpredictable. The αβ-mRNA is an α-type mRNA in which exon 4β remains unspliced, while the βγ-mRNA is a β-type. mRNA with the exon 4γ remaining unspliced. Recombinant enzymes expressed in Escherichia coli were all active and exhibited different enzymatic properties.

本文言語英語
ページ(範囲)677-688
ページ数12
ジャーナルJournal of biochemistry
112
5
DOI
出版ステータス出版済み - 11 1992
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学

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