Generation of a rat monoclonal antibody specific for Chd2

Akihito Harada, Saori Yoshimura, Jun Odawara, Masayuki Azuma, Seiji Okada, Mako Nakamura, Taro Tachibana, Yasuyuki Ohkawa

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

CHD2 is a member of the CHD family that contains chromodomain, helicase domain as well as DNA-binding domain. The CHD family is involved in gene expression and transcription by ATP-dependent chromatin remodeling. Analysis of mutant mouse revealed that CHD2 is involved in development as well as hematopoiesis, which suggests the involvement of CHD2 in gene expression. However, CHD2 has not yet been analyzed biochemically as there is no specific antibody against it. Here, we report on the establishment of specific monoclonal antibody (MAb) against CHD2 utilizing a rat medial iliac lymph node method. Through cell immunostaining utilizing established MAb to CHD2, we confirmed that CHD2 was localized in euchromatin. Additionally, IP-Western revealed that the expression level of full-length CHD2 did not change during the differentiation stage. Additionally, a specific signal was confirmed around 95 kDa at the undifferentiated stage. This clearly indicated that CHD2 was involved in specific gene expression at this stage. Thus, this antibody can contribute to elucidating the function of CHD2 in cell expression.

元の言語英語
ページ(範囲)173-177
ページ数5
ジャーナルHybridoma
29
発行部数2
DOI
出版物ステータス出版済み - 4 1 2010

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Monoclonal Antibodies
Gene Expression
Euchromatin
Chromatin Assembly and Disassembly
Antibodies
Hematopoiesis
Adenosine Triphosphate
Lymph Nodes
DNA

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

これを引用

Generation of a rat monoclonal antibody specific for Chd2. / Harada, Akihito; Yoshimura, Saori; Odawara, Jun; Azuma, Masayuki; Okada, Seiji; Nakamura, Mako; Tachibana, Taro; Ohkawa, Yasuyuki.

:: Hybridoma, 巻 29, 番号 2, 01.04.2010, p. 173-177.

研究成果: ジャーナルへの寄稿記事

Harada, Akihito ; Yoshimura, Saori ; Odawara, Jun ; Azuma, Masayuki ; Okada, Seiji ; Nakamura, Mako ; Tachibana, Taro ; Ohkawa, Yasuyuki. / Generation of a rat monoclonal antibody specific for Chd2. :: Hybridoma. 2010 ; 巻 29, 番号 2. pp. 173-177.
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AU - Harada, Akihito

AU - Yoshimura, Saori

AU - Odawara, Jun

AU - Azuma, Masayuki

AU - Okada, Seiji

AU - Nakamura, Mako

AU - Tachibana, Taro

AU - Ohkawa, Yasuyuki

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