Generation of biohybrid implants using a multipotent human periodontal ligament cell line and bioactive core materials

Taiga Ono, Atsushi Tomokiyo, Keita Ipposhi, Kozue Yamashita, M. Anas Alhasan, Yudai Miyazaki, Yoshihiro Kunitomi, Akira Tsuchiya, Kunio Ishikawa, Hidefumi Maeda

研究成果: Contribution to journalArticle査読

抄録

We aimed to generate periodontal ligament (PDL) tissue-like structures from a multipotent human PDL cell line using three-dimensional (3D) bioprinting technology and to incorporate these structures with bioactive core materials to develop a new biohybrid implant system. After 3D bioprinting, single-cell spheroids were able to form 3D tubular structures (3DTBs). We established three types of complexes using 3DTBs and different core materials: 3DTB-titanium core (TIC), 3DTB-hydroxyapatite core (HAC), and 3DTB without a core material (WOC). The expressions of PDL-, angiogenesis-, cementum-, and bone-related genes were significantly increased in the three complexes compared with monolayer-cultured cells. Abundant collagen fibers and cells positive for the above markers were confirmed in the three complexes. However, more positive cells were detected in HAC than in WOC or TIC. The present results suggest that 3D-bioprinted structures and hydroxyapatite core materials can function similarly to the PDL and may be useful for the development of a new biohybrid implant system.

本文言語英語
ページ(範囲)6742-6753
ページ数12
ジャーナルJournal of cellular physiology
236
9
DOI
出版ステータス出版済み - 9 2021

All Science Journal Classification (ASJC) codes

  • 生理学
  • 臨床生化学
  • 細胞生物学

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