Generation of Human Fatty Livers Using Custom-Engineered Induced Pluripotent Stem Cells with Modifiable SIRT1 Metabolism

Alexandra Collin de l'Hortet, Kazuki Takeishi, J. Guzman-Lepe, Kazutoyo Morita, Abhinav Achreja, Branimir Popovic, Yang Wang, Kan Handa, Anjali Mittal, Noah Meurs, Z. Zhu, F. Weinberg, Michael Salomon, I. J. Fox, Chu Xia Deng, Deepak Nagrath, Alejandro Soto-Gutierrez

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

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The mechanisms by which steatosis of the liver progresses to non-alcoholic steatohepatitis and end-stage liver disease remain elusive. Metabolic derangements in hepatocytes controlled by SIRT1 play a role in the development of fatty liver in inbred animals. The ability to perform similar studies using human tissue has been limited by the genetic variability in man. We generated human induced pluripotent stem cells (iPSCs) with controllable expression of SIRT1. By differentiating edited iPSCs into hepatocytes and knocking down SIRT1, we found increased fatty acid biosynthesis that exacerbates fat accumulation. To model human fatty livers, we repopulated decellularized rat livers with human mesenchymal cells, fibroblasts, macrophages, and human SIRT1 knockdown iPSC-derived hepatocytes and found that the human iPSC-derived liver tissue developed macrosteatosis, acquired proinflammatory phenotype, and shared a similar lipid and metabolic profiling to human fatty livers. Biofabrication of genetically edited human liver tissue may become an important tool for investigating human liver biology and disease. Collin de l'Hortet et al. biofabricated human fatty livers using genetically modified human hepatocytes differentiated from induced pluripotent stem cells, mesenchymal cells, fibroblasts, and macrophages. This methodology uncovered the molecular mechanisms of downregulated SIRT1 in human liver tissue. The biofabricated tissue reflected many aspects of human livers with non-alcoholic fatty liver disease.

元の言語英語
ページ(範囲)385-401.e9
ジャーナルCell metabolism
30
発行部数2
DOI
出版物ステータス出版済み - 8 6 2019

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Physiology
  • Molecular Biology
  • Cell Biology

これを引用

Collin de l'Hortet, A., Takeishi, K., Guzman-Lepe, J., Morita, K., Achreja, A., Popovic, B., ... Soto-Gutierrez, A. (2019). Generation of Human Fatty Livers Using Custom-Engineered Induced Pluripotent Stem Cells with Modifiable SIRT1 Metabolism. Cell metabolism, 30(2), 385-401.e9. https://doi.org/10.1016/j.cmet.2019.06.017