Generation of oocytes from mouse ES/iPS cells

Katsuhiko Hayashi, Mitinori Saitou

研究成果: ジャーナルへの寄稿記事

抄録

One of the paramount goals in reproductive biology is to produce functional oocytes in culture through a series of differentiation processes that accurately mimic those in vivo. Such a culture system would provide a larger number of oocytes than those available in vivo, which would be of help in the elucidation of mechanisms underlying germ cell development. All germ cells originate from a small group of primordial germ cells (PGCs) that segregate from somatic cell lineages at an early developmental stage. Recently, we developed a culture system in which embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) differentiate into PGC-like cells (PGCLCs) through an epiblast-like cell population. PGCLCs are capable of differentiating into functional oocytes when transplanted into the ovarian bursa with E12.5 gonadal somatic cells. In this review, we introduce our differentiation method and discuss possible applications of this culture system.

元の言語英語
ページ(範囲)70-78
ページ数9
ジャーナルJournal of Mammalian Ova Research
31
発行部数3
DOI
出版物ステータス出版済み - 1 1 2014

Fingerprint

Germ Cells
Oocytes
Germ Layers
Induced Pluripotent Stem Cells
Cell Lineage
Embryonic Stem Cells
Population

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Cell Biology

これを引用

Generation of oocytes from mouse ES/iPS cells. / Hayashi, Katsuhiko; Saitou, Mitinori.

:: Journal of Mammalian Ova Research, 巻 31, 番号 3, 01.01.2014, p. 70-78.

研究成果: ジャーナルへの寄稿記事

Hayashi, Katsuhiko ; Saitou, Mitinori. / Generation of oocytes from mouse ES/iPS cells. :: Journal of Mammalian Ova Research. 2014 ; 巻 31, 番号 3. pp. 70-78.
@article{3feb275abb6c461ea6f5edf9ebf249e1,
title = "Generation of oocytes from mouse ES/iPS cells",
abstract = "One of the paramount goals in reproductive biology is to produce functional oocytes in culture through a series of differentiation processes that accurately mimic those in vivo. Such a culture system would provide a larger number of oocytes than those available in vivo, which would be of help in the elucidation of mechanisms underlying germ cell development. All germ cells originate from a small group of primordial germ cells (PGCs) that segregate from somatic cell lineages at an early developmental stage. Recently, we developed a culture system in which embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) differentiate into PGC-like cells (PGCLCs) through an epiblast-like cell population. PGCLCs are capable of differentiating into functional oocytes when transplanted into the ovarian bursa with E12.5 gonadal somatic cells. In this review, we introduce our differentiation method and discuss possible applications of this culture system.",
author = "Katsuhiko Hayashi and Mitinori Saitou",
year = "2014",
month = "1",
day = "1",
doi = "10.1274/jmor.31.70",
language = "English",
volume = "31",
pages = "70--78",
journal = "Journal of Mammalian Ova Research",
issn = "1341-7738",
number = "3",

}

TY - JOUR

T1 - Generation of oocytes from mouse ES/iPS cells

AU - Hayashi, Katsuhiko

AU - Saitou, Mitinori

PY - 2014/1/1

Y1 - 2014/1/1

N2 - One of the paramount goals in reproductive biology is to produce functional oocytes in culture through a series of differentiation processes that accurately mimic those in vivo. Such a culture system would provide a larger number of oocytes than those available in vivo, which would be of help in the elucidation of mechanisms underlying germ cell development. All germ cells originate from a small group of primordial germ cells (PGCs) that segregate from somatic cell lineages at an early developmental stage. Recently, we developed a culture system in which embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) differentiate into PGC-like cells (PGCLCs) through an epiblast-like cell population. PGCLCs are capable of differentiating into functional oocytes when transplanted into the ovarian bursa with E12.5 gonadal somatic cells. In this review, we introduce our differentiation method and discuss possible applications of this culture system.

AB - One of the paramount goals in reproductive biology is to produce functional oocytes in culture through a series of differentiation processes that accurately mimic those in vivo. Such a culture system would provide a larger number of oocytes than those available in vivo, which would be of help in the elucidation of mechanisms underlying germ cell development. All germ cells originate from a small group of primordial germ cells (PGCs) that segregate from somatic cell lineages at an early developmental stage. Recently, we developed a culture system in which embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) differentiate into PGC-like cells (PGCLCs) through an epiblast-like cell population. PGCLCs are capable of differentiating into functional oocytes when transplanted into the ovarian bursa with E12.5 gonadal somatic cells. In this review, we introduce our differentiation method and discuss possible applications of this culture system.

UR - http://www.scopus.com/inward/record.url?scp=84916603104&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84916603104&partnerID=8YFLogxK

U2 - 10.1274/jmor.31.70

DO - 10.1274/jmor.31.70

M3 - Article

AN - SCOPUS:84916603104

VL - 31

SP - 70

EP - 78

JO - Journal of Mammalian Ova Research

JF - Journal of Mammalian Ova Research

SN - 1341-7738

IS - 3

ER -