Generation of specificity-variant antibodies by alteration of carbohydrate in light chain of human monoclonal antibodies

Hirofumi Tachibana, Sanetaka Shirahata, Hiroki Murakami

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

A hybridoma line, C5TN, produced human monoclonal antibody of which light chain had N-linked carbohydrate chain within the variable region. Some molecular-weight variants of light chain of the antibody were produced by C5TN variants resistant to cytotoxic effect of concanavalin A. The variant antibodies significantly altered the original cross-reactivity with antigens or lost the ability of antigen binding. The variants variously trimmed their carbohydrate chains by glycosidases, showed the changed reactivity or acquired the ability to bind for antigens. The carbohydrate-deficient antibodies from tunicamycin-treated C5TN and the variant clones behaved in a similar manner on antigen-binding reactivity. Furthermore, comparison of antibodies of which light chains have carbohydrate chains sensitive and resistant to some glycosidases showed that carbohydrate chain in variable region of light chain can influence their reactivity with antigen.

元の言語英語
ページ(範囲)625-632
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
189
発行部数2
DOI
出版物ステータス出版済み - 12 15 1992

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Antibody Specificity
Monoclonal Antibodies
Carbohydrates
Light
Antigens
Antibodies
Glycoside Hydrolases
Tunicamycin
Hybridomas
Concanavalin A
Clone Cells
Molecular Weight
Molecular weight

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Generation of specificity-variant antibodies by alteration of carbohydrate in light chain of human monoclonal antibodies. / Tachibana, Hirofumi; Shirahata, Sanetaka; Murakami, Hiroki.

:: Biochemical and Biophysical Research Communications, 巻 189, 番号 2, 15.12.1992, p. 625-632.

研究成果: ジャーナルへの寄稿記事

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