Generation of the Rat Monoclonal Antibody against the Extracellular Domain of Human CD63 by DNA Immunization

Liu Xu, Kan Ichiro Ihara, Saori Yoshimura, Daijiro Konno, Akira Tachibana, Takeshi Nakanishi, Taro Tachibana

研究成果: Contribution to journalArticle査読

抄録

Human cluster of differentiation 63 (hCD63) is one of the tetraspanin receptors that is abundant on the surface of exosomes. Exosomes are involved in cell-to-cell communication, including from cancer cells to normal cells. It is very important to detect exosomes as a marker for the diagnosis of various diseases. In this study, we report the generation and characterization of a monoclonal antibody (mAb) against the extracellular domain of hCD63 using DNA immunization. This mAb, clone 1C8-2B11, exhibits high performance for use in immunofluorescence and flow cytometry, and it has 10-fold higher affinity than the control antibody that is commercially available. mAb 1C8-2B11 has great potential to be a tool for research and clinical diagnosis.

本文言語英語
ページ(範囲)74-76
ページ数3
ジャーナルMonoclonal Antibodies in Immunodiagnosis and Immunotherapy
39
3
DOI
出版ステータス出版済み - 6 2020

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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