Generation of transgenic mice producing a human transthyretin variant: A possible mouse model for familial amyloidotic polyneuropathy

Hiroyuki Sasaki, Shigenobu Toné, Masamitsu Nakazato, Katsuji Yoshioka, Hisayuki Matsuo, Yoshihiro Kato, Yoshiyuki Sakaki

研究成果: ジャーナルへの寄稿記事

16 引用 (Scopus)

抄録

Type I familial amyloidotic polyneuropathy(FAP) results from the systemic deposition of a plasma transthyretin(TTR) variant with a Val ▶ Met change at position 30. In an attempt to establish a model of this disease, we generated transgenic mice producing the variant TIR. A DNA fragment containing the mouse metallothionein-I promoter fused to the structural gene coding for the human TTR variant was microinjected into fertilized mouse eggs. Among 72 mice that developed from these eggs, ten carried the fusion gene and three of these showed significant concentrations of the variant TTR in their serum. These mice may be useful in elucidating the pathogenesis of FAP and in establishing a therapy for this intractable disorder.

元の言語英語
ページ(範囲)794-799
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
139
発行部数2
DOI
出版物ステータス出版済み - 9 16 1986

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Prealbumin
Polyneuropathies
Transgenic Mice
Genes
Metallothionein
Zygote
Gene Fusion
Fusion reactions
Eggs
Plasmas
DNA
Serum
Therapeutics

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Generation of transgenic mice producing a human transthyretin variant : A possible mouse model for familial amyloidotic polyneuropathy. / Sasaki, Hiroyuki; Toné, Shigenobu; Nakazato, Masamitsu; Yoshioka, Katsuji; Matsuo, Hisayuki; Kato, Yoshihiro; Sakaki, Yoshiyuki.

:: Biochemical and Biophysical Research Communications, 巻 139, 番号 2, 16.09.1986, p. 794-799.

研究成果: ジャーナルへの寄稿記事

Sasaki, Hiroyuki ; Toné, Shigenobu ; Nakazato, Masamitsu ; Yoshioka, Katsuji ; Matsuo, Hisayuki ; Kato, Yoshihiro ; Sakaki, Yoshiyuki. / Generation of transgenic mice producing a human transthyretin variant : A possible mouse model for familial amyloidotic polyneuropathy. :: Biochemical and Biophysical Research Communications. 1986 ; 巻 139, 番号 2. pp. 794-799.
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