TY - JOUR
T1 - Genes encoding small heat shock proteins of the silkworm, Bombyx mori
AU - Sakano, Daisuke
AU - Li, Bin
AU - Xia, Qingyou
AU - Yamamoto, Kohji
AU - Fujii, Hiroshi
AU - Aso, Yoichi
N1 - Funding Information:
We are grateful to Dr. K. Mita of the National Institute of Agrobiological Sciences and Dr. T. Shimada of Tokyo University for supplying plasmid DNA. This study was supported by the National Bio-Resources Project (Silkworm) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2006
Y1 - 2006
N2 - Small heat shock protein (sHSP) is a family of ubiquitous polypeptides involved in a variety of physiological phenomena. From the silkworm, Bombyx mori, we isolated and sequenced the following cDNAs encoding sHSPs: shsp19.9, shsp20.1, shsp20.4, shsp20.8, shsp21.4, and shsp23.7. shsp21.4 was nearly twice as large in size as other shsps. The deduced amino acid sequence of sHSP21.4 was similar to that of Drosophila melanogaster CG14207-PA. Other sHSPs were highly similar to each other and, in a phylogenetic tree, formed a cluster including Plodia interpunctella αCP25. It was speculated that shsp21.4 has at least one intron in genome while other shsps do not. The transcripts of all shsps were subtle, but were constitutively detected in various tissues. Heat shock triggered a substantial increase in the transcripts other than shsp21.4. The B. mori sHSPs are perhaps classified into at least two groups: sHSP21.4 and others.
AB - Small heat shock protein (sHSP) is a family of ubiquitous polypeptides involved in a variety of physiological phenomena. From the silkworm, Bombyx mori, we isolated and sequenced the following cDNAs encoding sHSPs: shsp19.9, shsp20.1, shsp20.4, shsp20.8, shsp21.4, and shsp23.7. shsp21.4 was nearly twice as large in size as other shsps. The deduced amino acid sequence of sHSP21.4 was similar to that of Drosophila melanogaster CG14207-PA. Other sHSPs were highly similar to each other and, in a phylogenetic tree, formed a cluster including Plodia interpunctella αCP25. It was speculated that shsp21.4 has at least one intron in genome while other shsps do not. The transcripts of all shsps were subtle, but were constitutively detected in various tissues. Heat shock triggered a substantial increase in the transcripts other than shsp21.4. The B. mori sHSPs are perhaps classified into at least two groups: sHSP21.4 and others.
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U2 - 10.1271/bbb.60176
DO - 10.1271/bbb.60176
M3 - Article
C2 - 17031055
AN - SCOPUS:33750447637
VL - 70
SP - 2443
EP - 2450
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 10
ER -