Genetic analysis of cysteine-poor prolamin polypeptides reduced in the endosperm of the rice esp1 mutant

Tomokazu Ushijima, Hiroaki Matsusaka, Hiroyuki Jikuya, Masahiro Ogawa, Hikaru Satoh, Toshihiro Kumamaru

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

The esp1 mutant CM21 specifically exhibits reduced levels of cysteine-poor (CysP) prolamin bands with pIs of 6.65, 6.95, 7.10, and 7.35 in rice seed. Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis demonstrated that the bands with pIs 6.65, 6.95, and 7.35 are encoded by different structural genes. These results suggest that the Esp1 locus encodes a regulatory factor involved in the synthesis and/or accumulation of CysP prolamin molecules. Isoelectric focusing (IEF) analysis of CysP prolamins in chromosome substitution lines showed that structural genes for bands with pI values of 6.95, 7.10, and 7.35, which are reduced in esp1 mutant lines, are located as a gene cluster in the 44.2. cM region on chromosome 5.

元の言語英語
ページ(範囲)125-131
ページ数7
ジャーナルPlant Science
181
発行部数2
DOI
出版物ステータス出版済み - 8 1 2011

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Prolamins
Endosperm
prolamins
endosperm
Cysteine
cysteine
genetic techniques and protocols
polypeptides
structural genes
rice
mutants
Peptides
Chromosomes, Human, Pair 5
substitution lines
matrix-assisted laser desorption-ionization mass spectrometry
isoelectric focusing
Isoelectric Focusing
Multigene Family
multigene family
Genes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

これを引用

Genetic analysis of cysteine-poor prolamin polypeptides reduced in the endosperm of the rice esp1 mutant. / Ushijima, Tomokazu; Matsusaka, Hiroaki; Jikuya, Hiroyuki; Ogawa, Masahiro; Satoh, Hikaru; Kumamaru, Toshihiro.

:: Plant Science, 巻 181, 番号 2, 01.08.2011, p. 125-131.

研究成果: ジャーナルへの寄稿記事

Ushijima, Tomokazu ; Matsusaka, Hiroaki ; Jikuya, Hiroyuki ; Ogawa, Masahiro ; Satoh, Hikaru ; Kumamaru, Toshihiro. / Genetic analysis of cysteine-poor prolamin polypeptides reduced in the endosperm of the rice esp1 mutant. :: Plant Science. 2011 ; 巻 181, 番号 2. pp. 125-131.
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abstract = "The esp1 mutant CM21 specifically exhibits reduced levels of cysteine-poor (CysP) prolamin bands with pIs of 6.65, 6.95, 7.10, and 7.35 in rice seed. Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis demonstrated that the bands with pIs 6.65, 6.95, and 7.35 are encoded by different structural genes. These results suggest that the Esp1 locus encodes a regulatory factor involved in the synthesis and/or accumulation of CysP prolamin molecules. Isoelectric focusing (IEF) analysis of CysP prolamins in chromosome substitution lines showed that structural genes for bands with pI values of 6.95, 7.10, and 7.35, which are reduced in esp1 mutant lines, are located as a gene cluster in the 44.2. cM region on chromosome 5.",
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AU - Ushijima, Tomokazu

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AU - Jikuya, Hiroyuki

AU - Ogawa, Masahiro

AU - Satoh, Hikaru

AU - Kumamaru, Toshihiro

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AB - The esp1 mutant CM21 specifically exhibits reduced levels of cysteine-poor (CysP) prolamin bands with pIs of 6.65, 6.95, 7.10, and 7.35 in rice seed. Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis demonstrated that the bands with pIs 6.65, 6.95, and 7.35 are encoded by different structural genes. These results suggest that the Esp1 locus encodes a regulatory factor involved in the synthesis and/or accumulation of CysP prolamin molecules. Isoelectric focusing (IEF) analysis of CysP prolamins in chromosome substitution lines showed that structural genes for bands with pI values of 6.95, 7.10, and 7.35, which are reduced in esp1 mutant lines, are located as a gene cluster in the 44.2. cM region on chromosome 5.

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