Genetic analysis of the cell binding domain region of the chicken fibronectin gene

Shigeo Kubomura, Masanobu Obara, Yuji Karasaki, Hatsumi Taniguchi, Sadao Gotoh, Tohru Tsuda, Ken Higashi, Keiichi Ohsato, Hideyasu Hirano

研究成果: Contribution to journalArticle査読

11 被引用数 (Scopus)

抄録

We have determined the nucleotide sequence of the cell binding domain region of the chicken fibronectin gene and analyzed it evolutionaly. We present here the complete nucleotide sequence of 4.3 kb HindIII/EcoRI segment from the clone λFC23 of the chicken fibronectin gene. There were five exons in this segment. When we lined up the amino acid of exons 28, 29 and 31, three alignments, known as the Type III repeat, appeared. Tetrapeptide, -RGDS-, called the cell binding domain, existed in the second repeat, coding exon 30. It was presumed that the Type III repeats were composed of two exons in the chicken gene, the same as in the rat and humans. We found repeatedly appearing amino-acid sequences such as -TIT- (three arrays in these Type III repeats) but also found one of the amino acids substituted in the tripeptide in these Type III repeats (seven arrays). We analyzed these repeats from the point of view of evolution. We used three of the nucleotide sequences (12-18 bp) coding such -TIT- repeats as a unit length for comparing the various homologies after dividing the coding region into 56 segments. The mutual homology of the divided segments to each one of three showed 53% on average. On the other hand, the mutual nucleotide homology of the Type III repeat was 44%. This suggested that the Type III repeat may have been developed by frequent duplication of small gene units.

本文言語英語
ページ(範囲)171-181
ページ数11
ジャーナルBBA - Gene Structure and Expression
910
2
DOI
出版ステータス出版済み - 11 20 1987
外部発表はい

All Science Journal Classification (ASJC) codes

  • 構造生物学
  • 生物理学
  • 生化学
  • 遺伝学

フィンガープリント

「Genetic analysis of the cell binding domain region of the chicken fibronectin gene」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル