Genetic analysis of the gene cluster for the synthesis of serotype a-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans

Nao Suzuki, Yoshio Nakano, Yasuo Yoshida, Hiroshi Nakao, Yoshihisa Yamashita, Toshihiko Koga

研究成果: Contribution to journalArticle査読

24 被引用数 (Scopus)

抄録

The serotype a-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans consists of 6-deoxy-D-talose. A gene cluster associated with the biosynthesis of SPA was cloned and sequenced from the chromosomal DNA of A. actinomycetemcomitans SUNYaB 75 (serotype a). This cluster consisted of 14 open reading frames. Insertional inactivation of eight genes in this cluster resulted in loss of the ability of A. actinomycetemcomitans SUNYaB 75 cells to produce the polysaccharide. A protein database search revealed that the 11 sequential genes containing these eight genes were not found in SPA-associated gene clusters of the other serotypes of A. actinomycetemcomitans. These results suggest that the gene cluster is unique to serotype a and is essential to the synthesis of the SPA. (C) 2000 Elsevier Science B.V.

本文言語英語
ページ(範囲)135-138
ページ数4
ジャーナルBiochimica et Biophysica Acta - Gene Structure and Expression
1517
1
DOI
出版ステータス出版済み - 12 15 2000

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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