Genetic analysis of the gene cluster responsible for synthesis of serotype e-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans

Yasuo Yoshida, Yoshio Nakano, Nao Suzuki, Hiroshi Nakao, Yoshihisa Yamashita, Toshihiko Koga

研究成果: ジャーナルへの寄稿学術誌査読

17 被引用数 (Scopus)

抄録

A gene cluster associated with the biosynthesis of the serotype e-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans IDH1705 belonging to serotype e was cloned and sequenced. This cluster consisted of 18 open reading frames. Escherichia coli produced the polysaccharide that reacts with the serotype e-specific antiserum when transformed with a plasmid containing the cluster. Comparing the structure of the gene cluster with similar clusters from A. actinomycetemcomitans strains Y4 (serotype b) and NCTC9710 (serotype c) revealed that a 5.3-kb region containing the distal half of one gene and two entire genes in the cluster from strain IDH1705 replaced a 6.2-kb region containing eight genes in the cluster from strain Y4, and a 4.7-kb region containing four genes in the cluster from strain NCTC9710. These results suggest that this region is essential to the antigenic specificity of serotype e A. actinomycetemcomitans. Copyright (C) 1999 Elsevier Science B.V.

本文言語英語
ページ(範囲)457-461
ページ数5
ジャーナルBiochimica et Biophysica Acta - Gene Structure and Expression
1489
2-3
DOI
出版ステータス出版済み - 12月 23 1999

!!!All Science Journal Classification (ASJC) codes

  • 構造生物学
  • 生物理学
  • 生化学
  • 遺伝学

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