TY - JOUR
T1 - Genetic analysis of the gene cluster responsible for synthesis of serotype e-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans
AU - Yoshida, Yasuo
AU - Nakano, Yoshio
AU - Suzuki, Nao
AU - Nakao, Hiroshi
AU - Yamashita, Yoshihisa
AU - Koga, Toshihiko
N1 - Funding Information:
This work was supported in part by Grants-in-Aid of Scientific Research (A) 10307054 (T.K.) and (B) 11470452 (T.K.) and a Grant-in-Aid for Encouragement of Young Scientists 11771157 (Y.N.) from the Ministry of Education, Science, Sports and Culture, Tokyo, Japan.
PY - 1999/12/23
Y1 - 1999/12/23
N2 - A gene cluster associated with the biosynthesis of the serotype e-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans IDH1705 belonging to serotype e was cloned and sequenced. This cluster consisted of 18 open reading frames. Escherichia coli produced the polysaccharide that reacts with the serotype e-specific antiserum when transformed with a plasmid containing the cluster. Comparing the structure of the gene cluster with similar clusters from A. actinomycetemcomitans strains Y4 (serotype b) and NCTC9710 (serotype c) revealed that a 5.3-kb region containing the distal half of one gene and two entire genes in the cluster from strain IDH1705 replaced a 6.2-kb region containing eight genes in the cluster from strain Y4, and a 4.7-kb region containing four genes in the cluster from strain NCTC9710. These results suggest that this region is essential to the antigenic specificity of serotype e A. actinomycetemcomitans. Copyright (C) 1999 Elsevier Science B.V.
AB - A gene cluster associated with the biosynthesis of the serotype e-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans IDH1705 belonging to serotype e was cloned and sequenced. This cluster consisted of 18 open reading frames. Escherichia coli produced the polysaccharide that reacts with the serotype e-specific antiserum when transformed with a plasmid containing the cluster. Comparing the structure of the gene cluster with similar clusters from A. actinomycetemcomitans strains Y4 (serotype b) and NCTC9710 (serotype c) revealed that a 5.3-kb region containing the distal half of one gene and two entire genes in the cluster from strain IDH1705 replaced a 6.2-kb region containing eight genes in the cluster from strain Y4, and a 4.7-kb region containing four genes in the cluster from strain NCTC9710. These results suggest that this region is essential to the antigenic specificity of serotype e A. actinomycetemcomitans. Copyright (C) 1999 Elsevier Science B.V.
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U2 - 10.1016/S0167-4781(99)00192-X
DO - 10.1016/S0167-4781(99)00192-X
M3 - Article
C2 - 10673051
AN - SCOPUS:0032758724
VL - 1489
SP - 457
EP - 461
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 2-3
ER -