Genetic polymorphisms of OCT-1 confer susceptibility to severe progression of primary biliary cirrhosis in Japanese patients

Yuki Ohishi, Makoto Nakamuta, Naoko Ishikawa, Ohki Saitoh, Hitomi Nakamura, Yoshihiro Aiba, Atsumasa Komori, Kiyoshi Migita, Hiroshi Yatsuhashi, Nobuyoshi Fukushima, Motoyuki Kohjima, Tsuyoshi Yoshimoto, Kunitaka Fukuizumi, Makoto Ishibashi, Takashi Nishino, Ken Shirabe, Akinobu Taketomi, Yoshihiko Maehara, Hiromi Ishibashi, Minoru Nakamura

研究成果: ジャーナルへの寄稿記事

16 引用 (Scopus)

抄録

Background: To identify the genetic factors involved in the pathogenesis of primary biliary cirrhosis (PBC), we focused on the organic cation transporter 1 (OCT1/SLC22A1), which is closely associated with phosphatidylcholine synthesis in hepatocytes. Methods: We selected four (rs683369, rs2282143, rs622342 and rs1443844) OCT-1 single nucleotide polymorphisms (SNPs), and genotyped these SNPs using the TaqMan probe method in 275 Japanese PBC patients and 194 gender-matched, healthy volunteers as controls. Results: The Chi-square test revealed that the rs683369 variant allele (G) was associated with insusceptibility to PBC development [P = 0.009, odds ratio (OR) 0.60, 95 % confidence interval (CI) 0.40-0.88] in an allele model, and that the rs683369 variant allele (G) was associated with jaundice-type progression in a minor allele dominant genotype model (P = 0.032, OR 3.10, 95 % CI 1.05-9.14). The OCT-1 rs2282143 variant (T) and rs622342 variant (C) were also associated with jaundice-type progression in a minor allele recessive genotype model (P = 0.0002, OR 10.58, 95 % CI 2.36-47.54, and P = 0.006, OR 7.84, 95 % CI 1.39-44.36, respectively). Furthermore, the association of OCT-1 rs683369 and rs622342 with susceptibility to jaundice-type progression was confirmed by a replication study with a distinct set of PBC patients who underwent liver transplantation. Conclusions: The present study is the first report on the association of OCT-1 genetic polymorphisms with the overall development and jaundice-type progression of PBC.

元の言語英語
ページ(範囲)332-342
ページ数11
ジャーナルJournal of gastroenterology
49
発行部数2
DOI
出版物ステータス出版済み - 2 1 2014

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Biliary Liver Cirrhosis
Genetic Polymorphisms
Jaundice
Alleles
Odds Ratio
Confidence Intervals
Single Nucleotide Polymorphism
Organic Cation Transporter 1
Genotype
Chi-Square Distribution
Phosphatidylcholines
Liver Transplantation
Hepatocytes
Healthy Volunteers

All Science Journal Classification (ASJC) codes

  • Gastroenterology

これを引用

Genetic polymorphisms of OCT-1 confer susceptibility to severe progression of primary biliary cirrhosis in Japanese patients. / Ohishi, Yuki; Nakamuta, Makoto; Ishikawa, Naoko; Saitoh, Ohki; Nakamura, Hitomi; Aiba, Yoshihiro; Komori, Atsumasa; Migita, Kiyoshi; Yatsuhashi, Hiroshi; Fukushima, Nobuyoshi; Kohjima, Motoyuki; Yoshimoto, Tsuyoshi; Fukuizumi, Kunitaka; Ishibashi, Makoto; Nishino, Takashi; Shirabe, Ken; Taketomi, Akinobu; Maehara, Yoshihiko; Ishibashi, Hiromi; Nakamura, Minoru.

:: Journal of gastroenterology, 巻 49, 番号 2, 01.02.2014, p. 332-342.

研究成果: ジャーナルへの寄稿記事

Ohishi, Y, Nakamuta, M, Ishikawa, N, Saitoh, O, Nakamura, H, Aiba, Y, Komori, A, Migita, K, Yatsuhashi, H, Fukushima, N, Kohjima, M, Yoshimoto, T, Fukuizumi, K, Ishibashi, M, Nishino, T, Shirabe, K, Taketomi, A, Maehara, Y, Ishibashi, H & Nakamura, M 2014, 'Genetic polymorphisms of OCT-1 confer susceptibility to severe progression of primary biliary cirrhosis in Japanese patients', Journal of gastroenterology, 巻. 49, 番号 2, pp. 332-342. https://doi.org/10.1007/s00535-013-0795-0
Ohishi, Yuki ; Nakamuta, Makoto ; Ishikawa, Naoko ; Saitoh, Ohki ; Nakamura, Hitomi ; Aiba, Yoshihiro ; Komori, Atsumasa ; Migita, Kiyoshi ; Yatsuhashi, Hiroshi ; Fukushima, Nobuyoshi ; Kohjima, Motoyuki ; Yoshimoto, Tsuyoshi ; Fukuizumi, Kunitaka ; Ishibashi, Makoto ; Nishino, Takashi ; Shirabe, Ken ; Taketomi, Akinobu ; Maehara, Yoshihiko ; Ishibashi, Hiromi ; Nakamura, Minoru. / Genetic polymorphisms of OCT-1 confer susceptibility to severe progression of primary biliary cirrhosis in Japanese patients. :: Journal of gastroenterology. 2014 ; 巻 49, 番号 2. pp. 332-342.
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title = "Genetic polymorphisms of OCT-1 confer susceptibility to severe progression of primary biliary cirrhosis in Japanese patients",
abstract = "Background: To identify the genetic factors involved in the pathogenesis of primary biliary cirrhosis (PBC), we focused on the organic cation transporter 1 (OCT1/SLC22A1), which is closely associated with phosphatidylcholine synthesis in hepatocytes. Methods: We selected four (rs683369, rs2282143, rs622342 and rs1443844) OCT-1 single nucleotide polymorphisms (SNPs), and genotyped these SNPs using the TaqMan probe method in 275 Japanese PBC patients and 194 gender-matched, healthy volunteers as controls. Results: The Chi-square test revealed that the rs683369 variant allele (G) was associated with insusceptibility to PBC development [P = 0.009, odds ratio (OR) 0.60, 95 {\%} confidence interval (CI) 0.40-0.88] in an allele model, and that the rs683369 variant allele (G) was associated with jaundice-type progression in a minor allele dominant genotype model (P = 0.032, OR 3.10, 95 {\%} CI 1.05-9.14). The OCT-1 rs2282143 variant (T) and rs622342 variant (C) were also associated with jaundice-type progression in a minor allele recessive genotype model (P = 0.0002, OR 10.58, 95 {\%} CI 2.36-47.54, and P = 0.006, OR 7.84, 95 {\%} CI 1.39-44.36, respectively). Furthermore, the association of OCT-1 rs683369 and rs622342 with susceptibility to jaundice-type progression was confirmed by a replication study with a distinct set of PBC patients who underwent liver transplantation. Conclusions: The present study is the first report on the association of OCT-1 genetic polymorphisms with the overall development and jaundice-type progression of PBC.",
author = "Yuki Ohishi and Makoto Nakamuta and Naoko Ishikawa and Ohki Saitoh and Hitomi Nakamura and Yoshihiro Aiba and Atsumasa Komori and Kiyoshi Migita and Hiroshi Yatsuhashi and Nobuyoshi Fukushima and Motoyuki Kohjima and Tsuyoshi Yoshimoto and Kunitaka Fukuizumi and Makoto Ishibashi and Takashi Nishino and Ken Shirabe and Akinobu Taketomi and Yoshihiko Maehara and Hiromi Ishibashi and Minoru Nakamura",
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T1 - Genetic polymorphisms of OCT-1 confer susceptibility to severe progression of primary biliary cirrhosis in Japanese patients

AU - Ohishi, Yuki

AU - Nakamuta, Makoto

AU - Ishikawa, Naoko

AU - Saitoh, Ohki

AU - Nakamura, Hitomi

AU - Aiba, Yoshihiro

AU - Komori, Atsumasa

AU - Migita, Kiyoshi

AU - Yatsuhashi, Hiroshi

AU - Fukushima, Nobuyoshi

AU - Kohjima, Motoyuki

AU - Yoshimoto, Tsuyoshi

AU - Fukuizumi, Kunitaka

AU - Ishibashi, Makoto

AU - Nishino, Takashi

AU - Shirabe, Ken

AU - Taketomi, Akinobu

AU - Maehara, Yoshihiko

AU - Ishibashi, Hiromi

AU - Nakamura, Minoru

PY - 2014/2/1

Y1 - 2014/2/1

N2 - Background: To identify the genetic factors involved in the pathogenesis of primary biliary cirrhosis (PBC), we focused on the organic cation transporter 1 (OCT1/SLC22A1), which is closely associated with phosphatidylcholine synthesis in hepatocytes. Methods: We selected four (rs683369, rs2282143, rs622342 and rs1443844) OCT-1 single nucleotide polymorphisms (SNPs), and genotyped these SNPs using the TaqMan probe method in 275 Japanese PBC patients and 194 gender-matched, healthy volunteers as controls. Results: The Chi-square test revealed that the rs683369 variant allele (G) was associated with insusceptibility to PBC development [P = 0.009, odds ratio (OR) 0.60, 95 % confidence interval (CI) 0.40-0.88] in an allele model, and that the rs683369 variant allele (G) was associated with jaundice-type progression in a minor allele dominant genotype model (P = 0.032, OR 3.10, 95 % CI 1.05-9.14). The OCT-1 rs2282143 variant (T) and rs622342 variant (C) were also associated with jaundice-type progression in a minor allele recessive genotype model (P = 0.0002, OR 10.58, 95 % CI 2.36-47.54, and P = 0.006, OR 7.84, 95 % CI 1.39-44.36, respectively). Furthermore, the association of OCT-1 rs683369 and rs622342 with susceptibility to jaundice-type progression was confirmed by a replication study with a distinct set of PBC patients who underwent liver transplantation. Conclusions: The present study is the first report on the association of OCT-1 genetic polymorphisms with the overall development and jaundice-type progression of PBC.

AB - Background: To identify the genetic factors involved in the pathogenesis of primary biliary cirrhosis (PBC), we focused on the organic cation transporter 1 (OCT1/SLC22A1), which is closely associated with phosphatidylcholine synthesis in hepatocytes. Methods: We selected four (rs683369, rs2282143, rs622342 and rs1443844) OCT-1 single nucleotide polymorphisms (SNPs), and genotyped these SNPs using the TaqMan probe method in 275 Japanese PBC patients and 194 gender-matched, healthy volunteers as controls. Results: The Chi-square test revealed that the rs683369 variant allele (G) was associated with insusceptibility to PBC development [P = 0.009, odds ratio (OR) 0.60, 95 % confidence interval (CI) 0.40-0.88] in an allele model, and that the rs683369 variant allele (G) was associated with jaundice-type progression in a minor allele dominant genotype model (P = 0.032, OR 3.10, 95 % CI 1.05-9.14). The OCT-1 rs2282143 variant (T) and rs622342 variant (C) were also associated with jaundice-type progression in a minor allele recessive genotype model (P = 0.0002, OR 10.58, 95 % CI 2.36-47.54, and P = 0.006, OR 7.84, 95 % CI 1.39-44.36, respectively). Furthermore, the association of OCT-1 rs683369 and rs622342 with susceptibility to jaundice-type progression was confirmed by a replication study with a distinct set of PBC patients who underwent liver transplantation. Conclusions: The present study is the first report on the association of OCT-1 genetic polymorphisms with the overall development and jaundice-type progression of PBC.

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