Genetical studies on the N-methyl-N-nitrosourea induced non-molting b“ mutation in Bombyx mori”

Yutaka Banno, Yutaka Kawaguchi, Hiroshi Doira

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

Newly laid silkworm eggs of a inbred normal strain were immersed in 0.5mM N-methyl-N-nitrosourea solution for 60 minutes. The eggs were at an age of 90±15 minutes when the treatment was started. In the M3 progenies after the treatment, a considerable number of dwarf larva was found on the second day of the first larval instar. All dwarf larvae continued to eat mulberry leaves slightly increasing body-size for about ten days, and then died without entering into molt. Duration of the first larval instar is 3–4 days for normal development of this insect. Genetic analysis revealed that the dwarf and non-molting lethal character was controlled by an induced recessive mutation. The mutant was named “non-molting b” with gene symbol nm-b. Linkage experiment showed that nm-b was linked with plain (p) and Yellow-blood (Y) which had been mapped at 0.0 and 25.6 position, respectively, on ganetic map of the second linkage group. Precise localization of nm-b gene was made by a three-point experiment involving pM, Y and nm-b. The recombination value between pMand Y was calculated to be 25.68%, nm-b and Y 0.55%, and pMand Y 26.23%, Hence the arrangement of these three gene loci on the chromosome is in. the order of p-nm-b-Y. Taking correction factor into account, the locus of of nm-b is determined as 25.1 position on the second linkage group.

元の言語英語
ページ(範囲)227-231
ページ数5
ジャーナルJournal of Sericultural Science of Japan
54
発行部数3
DOI
出版物ステータス出版済み - 1985

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N-methyl-N-nitrosourea
Methylnitrosourea
Bombyx
Bombyx mori
Eggs
Larva
Genes
mutation
linkage groups
Morus
Mutation
Gene Order
Body Size
instars
Genetic Recombination
Insects
insect development
loci
genes
Chromosomes

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Polymers and Plastics

これを引用

Genetical studies on the N-methyl-N-nitrosourea induced non-molting b“ mutation in Bombyx mori”. / Banno, Yutaka; Kawaguchi, Yutaka; Doira, Hiroshi.

:: Journal of Sericultural Science of Japan, 巻 54, 番号 3, 1985, p. 227-231.

研究成果: ジャーナルへの寄稿記事

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abstract = "Newly laid silkworm eggs of a inbred normal strain were immersed in 0.5mM N-methyl-N-nitrosourea solution for 60 minutes. The eggs were at an age of 90±15 minutes when the treatment was started. In the M3 progenies after the treatment, a considerable number of dwarf larva was found on the second day of the first larval instar. All dwarf larvae continued to eat mulberry leaves slightly increasing body-size for about ten days, and then died without entering into molt. Duration of the first larval instar is 3–4 days for normal development of this insect. Genetic analysis revealed that the dwarf and non-molting lethal character was controlled by an induced recessive mutation. The mutant was named “non-molting b” with gene symbol nm-b. Linkage experiment showed that nm-b was linked with plain (p) and Yellow-blood (Y) which had been mapped at 0.0 and 25.6 position, respectively, on ganetic map of the second linkage group. Precise localization of nm-b gene was made by a three-point experiment involving pM, Y and nm-b. The recombination value between pMand Y was calculated to be 25.68{\%}, nm-b and Y 0.55{\%}, and pMand Y 26.23{\%}, Hence the arrangement of these three gene loci on the chromosome is in. the order of p-nm-b-Y. Taking correction factor into account, the locus of of nm-b is determined as 25.1 position on the second linkage group.",
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