Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging

Shigenori Inagaki, Hidekazu Tsutsui, Kazushi Suzuki, Masakazu Agetsuma, Yoshiyuki Arai, Yuka Jinno, Guirong Bai, Matthew J. Daniels, Yasushi Okamura, Tomoki Matsuda, Takeharu Nagai

研究成果: Contribution to journalArticle査読

33 被引用数 (Scopus)

抄録

We report development of the first genetically encoded bioluminescent indicator for membrane voltage called LOTUS-V. Since it is bioluminescent, imaging LOTUS-V does not require external light illumination. This allows bidirectional optogenetic control of cellular activity triggered by Channelrhodopsin2 and Halorhodopsin during voltage imaging. The other advantage of LOTUS-V is the robustness of a signal-to-background ratio (SBR) wherever it expressed, even in the specimens where autofluorescence from environment severely interferes fluorescence imaging. Through imaging of moving cardiomyocyte aggregates, we demonstrated the advantages of LOTUS-V in long-term imaging are attributable to the absence of phototoxicity, and photobleaching in bioluminescent imaging, combined with the ratiometric aspect of LOTUS-V design. Collectively LOTUS-V extends the scope of excitable cell control and simultaneous voltage phenotyping, which should enable applications in bioscience, medicine and pharmacology previously not possible.

本文言語英語
論文番号42398
ジャーナルScientific reports
7
DOI
出版ステータス出版済み - 2 13 2017
外部発表はい

All Science Journal Classification (ASJC) codes

  • General

フィンガープリント 「Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル