Genetically fused charged peptides induce rapid crystallization of proteins

K. Minamihata; K. Tsukamoto; M. Adachi; R. Shimizu; M. Mishina; R. Kuroki; T. Nagamune

doi:10.1039/c9cc09529b

Genetically fused charged peptides induce rapid crystallization of proteins

K. Minamihata, K. Tsukamoto, M. Adachi, R. Shimizu, M. Mishina, R. Kuroki, T. Nagamune

分子情報システム

研究成果: Contribution to journal › Article › 査読

1 被引用数 (Scopus)

抄録

We utilized electrostatic interaction to induce rapid crystallization of streptavidin. Simply mixing streptavidins possessing either a positively or negatively charged peptide at their C-terminus generated diffraction-quality crystals in a few hours. We modified the streptavidin crystals with fluorescent molecules using biotin, demonstrating the concept of protein crystals as functional biomaterials.

本文言語	英語
ページ（範囲）	3891-3894
ページ数	4
ジャーナル	Chemical Communications
巻	56
号	27
DOI	https://doi.org/10.1039/c9cc09529b
出版ステータス	出版済み - 4 7 2020

All Science Journal Classification (ASJC) codes

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

文献へのアクセス

10.1039/c9cc09529b

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引用スタイル

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AU - Adachi, M.

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AU - Kuroki, R.

AU - Nagamune, T.

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