TY - JOUR
T1 - Genome editing of pufferfish saxitoxin- and tetrodotoxin-binding protein type 2 in Takifugu rubripes
AU - Kato-Unoki, Yoko
AU - Takai, Yuki
AU - Kinoshita, Masato
AU - Mochizuki, Toshitaka
AU - Tatsuno, Ryohei
AU - Shimasaki, Yohei
AU - Oshima, Yuji
N1 - Funding Information:
The authors thank Dr. Shigeharu Kinoshita of Tokyo University, for donating the matured eggs and sperm. This work was partly funded by Japan Society for the Promotion of Science (JSPS) KAKENHI Grant-in-Aid no. 25660169 and 18H02280 .
PY - 2018/10
Y1 - 2018/10
N2 - The pufferfish saxitoxin- and tetrodotoxin-binding protein 2 (PSTBP2), which is involved in toxin accumulation, was knocked out in Takifugu rubripes embryos by using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 genome-editing technology. Treating the embryos with one of two single-guide RNA (sgRNA) resulted in mutation rates of 57.1% and 62.5%, respectively, as estimated using a heteroduplex mobility assay at 3 days postfertilization. Both sgRNAs might induced frameshift mutations that knocked out the T. rubripes PSTBP2.
AB - The pufferfish saxitoxin- and tetrodotoxin-binding protein 2 (PSTBP2), which is involved in toxin accumulation, was knocked out in Takifugu rubripes embryos by using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 genome-editing technology. Treating the embryos with one of two single-guide RNA (sgRNA) resulted in mutation rates of 57.1% and 62.5%, respectively, as estimated using a heteroduplex mobility assay at 3 days postfertilization. Both sgRNAs might induced frameshift mutations that knocked out the T. rubripes PSTBP2.
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U2 - 10.1016/j.toxicon.2018.08.001
DO - 10.1016/j.toxicon.2018.08.001
M3 - Article
C2 - 30170168
AN - SCOPUS:85052660409
VL - 153
SP - 58
EP - 61
JO - Toxicon
JF - Toxicon
SN - 0041-0101
ER -