Genome editing of pufferfish saxitoxin- and tetrodotoxin-binding protein type 2 in Takifugu rubripes

Yoko Kato-Unoki, Yuki Takai, Masato Kinoshita, Toshitaka Mochizuki, Ryohei Tatsuno, Yohei Shimasaki, Yuji Oshima

研究成果: Contribution to journalArticle査読

3 被引用数 (Scopus)

抄録

The pufferfish saxitoxin- and tetrodotoxin-binding protein 2 (PSTBP2), which is involved in toxin accumulation, was knocked out in Takifugu rubripes embryos by using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 genome-editing technology. Treating the embryos with one of two single-guide RNA (sgRNA) resulted in mutation rates of 57.1% and 62.5%, respectively, as estimated using a heteroduplex mobility assay at 3 days postfertilization. Both sgRNAs might induced frameshift mutations that knocked out the T. rubripes PSTBP2.

本文言語英語
ページ(範囲)58-61
ページ数4
ジャーナルToxicon
153
DOI
出版ステータス出版済み - 10 2018

All Science Journal Classification (ASJC) codes

  • 毒物学

フィンガープリント

「Genome editing of pufferfish saxitoxin- and tetrodotoxin-binding protein type 2 in Takifugu rubripes」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル