抜粋
The pufferfish saxitoxin- and tetrodotoxin-binding protein 2 (PSTBP2), which is involved in toxin accumulation, was knocked out in Takifugu rubripes embryos by using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 genome-editing technology. Treating the embryos with one of two single-guide RNA (sgRNA) resulted in mutation rates of 57.1% and 62.5%, respectively, as estimated using a heteroduplex mobility assay at 3 days postfertilization. Both sgRNAs might induced frameshift mutations that knocked out the T. rubripes PSTBP2.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 58-61 |
| ページ数 | 4 |
| ジャーナル | Toxicon |
| 巻 | 153 |
| DOI | |
| 出版物ステータス | 出版済み - 10 2018 |
All Science Journal Classification (ASJC) codes
- Toxicology
フィンガープリント Genome editing of pufferfish saxitoxin- and tetrodotoxin-binding protein type 2 in Takifugu rubripes' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。
これを引用
Kato-Unoki, Y., Takai, Y., Kinoshita, M., Mochizuki, T., Tatsuno, R., Shimasaki, Y., & Oshima, Y. (2018). Genome editing of pufferfish saxitoxin- and tetrodotoxin-binding protein type 2 in Takifugu rubripes. Toxicon, 153, 58-61. https://doi.org/10.1016/j.toxicon.2018.08.001