Genome editing of pufferfish saxitoxin- and tetrodotoxin-binding protein type 2 in Takifugu rubripes

Yoko Kato-Unoki, Yuki Takai, Masato Kinoshita, Toshitaka Mochizuki, Ryohei Tatsuno, Yohei Shimasaki, Yuji Oshima

研究成果: ジャーナルへの寄稿記事

抄録

The pufferfish saxitoxin- and tetrodotoxin-binding protein 2 (PSTBP2), which is involved in toxin accumulation, was knocked out in Takifugu rubripes embryos by using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 genome-editing technology. Treating the embryos with one of two single-guide RNA (sgRNA) resulted in mutation rates of 57.1% and 62.5%, respectively, as estimated using a heteroduplex mobility assay at 3 days postfertilization. Both sgRNAs might induced frameshift mutations that knocked out the T. rubripes PSTBP2.

元の言語英語
ページ(範囲)58-61
ページ数4
ジャーナルToxicon
153
DOI
出版物ステータス出版済み - 10 1 2018

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Clustered Regularly Interspaced Short Palindromic Repeats
Takifugu
Saxitoxin
Tetraodontiformes
Embryonic Structures
Guide RNA
Genes
Frameshift Mutation
Mutation Rate
Assays
Technology
Proteins
Gene Editing
tetrodotoxin-binding protein

All Science Journal Classification (ASJC) codes

  • Toxicology

これを引用

Genome editing of pufferfish saxitoxin- and tetrodotoxin-binding protein type 2 in Takifugu rubripes. / Kato-Unoki, Yoko; Takai, Yuki; Kinoshita, Masato; Mochizuki, Toshitaka; Tatsuno, Ryohei; Shimasaki, Yohei; Oshima, Yuji.

:: Toxicon, 巻 153, 01.10.2018, p. 58-61.

研究成果: ジャーナルへの寄稿記事

Kato-Unoki, Yoko ; Takai, Yuki ; Kinoshita, Masato ; Mochizuki, Toshitaka ; Tatsuno, Ryohei ; Shimasaki, Yohei ; Oshima, Yuji. / Genome editing of pufferfish saxitoxin- and tetrodotoxin-binding protein type 2 in Takifugu rubripes. :: Toxicon. 2018 ; 巻 153. pp. 58-61.
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