抄録
The pufferfish saxitoxin- and tetrodotoxin-binding protein 2 (PSTBP2), which is involved in toxin accumulation, was knocked out in Takifugu rubripes embryos by using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 genome-editing technology. Treating the embryos with one of two single-guide RNA (sgRNA) resulted in mutation rates of 57.1% and 62.5%, respectively, as estimated using a heteroduplex mobility assay at 3 days postfertilization. Both sgRNAs might induced frameshift mutations that knocked out the T. rubripes PSTBP2.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 58-61 |
| ページ数 | 4 |
| ジャーナル | Toxicon |
| 巻 | 153 |
| DOI | |
| 出版物ステータス | 出版済み - 10 1 2018 |
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All Science Journal Classification (ASJC) codes
- Toxicology
これを引用
Genome editing of pufferfish saxitoxin- and tetrodotoxin-binding protein type 2 in Takifugu rubripes. / Kato-Unoki, Yoko; Takai, Yuki; Kinoshita, Masato; Mochizuki, Toshitaka; Tatsuno, Ryohei; Shimasaki, Yohei; Oshima, Yuji.
：: Toxicon, 巻 153, 01.10.2018, p. 58-61.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Genome editing of pufferfish saxitoxin- and tetrodotoxin-binding protein type 2 in Takifugu rubripes
AU - Kato-Unoki, Yoko
AU - Takai, Yuki
AU - Kinoshita, Masato
AU - Mochizuki, Toshitaka
AU - Tatsuno, Ryohei
AU - Shimasaki, Yohei
AU - Oshima, Yuji
PY - 2018/10/1
Y1 - 2018/10/1
N2 - The pufferfish saxitoxin- and tetrodotoxin-binding protein 2 (PSTBP2), which is involved in toxin accumulation, was knocked out in Takifugu rubripes embryos by using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 genome-editing technology. Treating the embryos with one of two single-guide RNA (sgRNA) resulted in mutation rates of 57.1% and 62.5%, respectively, as estimated using a heteroduplex mobility assay at 3 days postfertilization. Both sgRNAs might induced frameshift mutations that knocked out the T. rubripes PSTBP2.
AB - The pufferfish saxitoxin- and tetrodotoxin-binding protein 2 (PSTBP2), which is involved in toxin accumulation, was knocked out in Takifugu rubripes embryos by using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 genome-editing technology. Treating the embryos with one of two single-guide RNA (sgRNA) resulted in mutation rates of 57.1% and 62.5%, respectively, as estimated using a heteroduplex mobility assay at 3 days postfertilization. Both sgRNAs might induced frameshift mutations that knocked out the T. rubripes PSTBP2.
UR - http://www.scopus.com/inward/record.url?scp=85052660409&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85052660409&partnerID=8YFLogxK
U2 - 10.1016/j.toxicon.2018.08.001
DO - 10.1016/j.toxicon.2018.08.001
M3 - Article
C2 - 30170168
AN - SCOPUS:85052660409
VL - 153
SP - 58
EP - 61
JO - Toxicon
JF - Toxicon
SN - 0041-0101
ER -