抄録
The brain and cardiac isoforms of anion exchanger 3 (AE3) are considered to use their own promoters for their expression. However, little is known as to how the alternative transcription initiation is regulated. As a first step for elucidating the regulation, we obtained a genomic gene of mouse AE3. The 19-kbp clone contains about 6 kbp of 5′ flanking region, 23 exons, and 22 introns. We have sequenced the whole region including introns and determined the intron-exon boundaries. Six amino acids are different from those deduced from the reported mouse AE3 cDNA. We measured a promoter activity of the 5′ flanking region of the exon 1 for a brain type isoform and that of the exon C1 for a cardiac type isoform. The upstream region of the exon C1 indeed showed a promoter activity in rat cardiomyoblastic H9C2 cells, rat pheochromocyotoma PC12 cells, and human HeLa cells whereas the 5′ flanking region of the exon 1 does not in HeLa cells, suggesting that the promoter for the cardiac type is rather ubiquitously active.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 251-255 |
| ページ数 | 5 |
| ジャーナル | Mitochondrial DNA |
| 巻 | 13 |
| 発行部数 | 5 |
| DOI | |
| 出版物ステータス | 出版済み - 12 1 2002 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
これを引用
Genomic cloning and promoter analysis of a mouse anion exchanger 3 (AE3) gene. / Iwaasa, Mitsutoshi; Tatewaki, Hideki; Ohno, Tetsuji; Okubo, Kenshi; Hamasaki, Naotaka; Kang, Dongchon.
：: Mitochondrial DNA, 巻 13, 番号 5, 01.12.2002, p. 251-255.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Genomic cloning and promoter analysis of a mouse anion exchanger 3 (AE3) gene
AU - Iwaasa, Mitsutoshi
AU - Tatewaki, Hideki
AU - Ohno, Tetsuji
AU - Okubo, Kenshi
AU - Hamasaki, Naotaka
AU - Kang, Dongchon
PY - 2002/12/1
Y1 - 2002/12/1
N2 - The brain and cardiac isoforms of anion exchanger 3 (AE3) are considered to use their own promoters for their expression. However, little is known as to how the alternative transcription initiation is regulated. As a first step for elucidating the regulation, we obtained a genomic gene of mouse AE3. The 19-kbp clone contains about 6 kbp of 5′ flanking region, 23 exons, and 22 introns. We have sequenced the whole region including introns and determined the intron-exon boundaries. Six amino acids are different from those deduced from the reported mouse AE3 cDNA. We measured a promoter activity of the 5′ flanking region of the exon 1 for a brain type isoform and that of the exon C1 for a cardiac type isoform. The upstream region of the exon C1 indeed showed a promoter activity in rat cardiomyoblastic H9C2 cells, rat pheochromocyotoma PC12 cells, and human HeLa cells whereas the 5′ flanking region of the exon 1 does not in HeLa cells, suggesting that the promoter for the cardiac type is rather ubiquitously active.
AB - The brain and cardiac isoforms of anion exchanger 3 (AE3) are considered to use their own promoters for their expression. However, little is known as to how the alternative transcription initiation is regulated. As a first step for elucidating the regulation, we obtained a genomic gene of mouse AE3. The 19-kbp clone contains about 6 kbp of 5′ flanking region, 23 exons, and 22 introns. We have sequenced the whole region including introns and determined the intron-exon boundaries. Six amino acids are different from those deduced from the reported mouse AE3 cDNA. We measured a promoter activity of the 5′ flanking region of the exon 1 for a brain type isoform and that of the exon C1 for a cardiac type isoform. The upstream region of the exon C1 indeed showed a promoter activity in rat cardiomyoblastic H9C2 cells, rat pheochromocyotoma PC12 cells, and human HeLa cells whereas the 5′ flanking region of the exon 1 does not in HeLa cells, suggesting that the promoter for the cardiac type is rather ubiquitously active.
UR - http://www.scopus.com/inward/record.url?scp=0036805792&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036805792&partnerID=8YFLogxK
U2 - 10.1080/1042517021000011654
DO - 10.1080/1042517021000011654
M3 - Article
C2 - 12592704
AN - SCOPUS:0036805792
VL - 13
SP - 251
EP - 255
JO - DNA Sequence - Journal of DNA Sequencing and Mapping
JF - DNA Sequence - Journal of DNA Sequencing and Mapping
SN - 1940-1736
IS - 5
ER -