Genomic structure and expression analysis of the gene encoding a silkworm basic Kunitz-type chymotrypsin inhibitor

Ningjia He, Masatoshi Yakiyama, Hiroshi Fujii, Yutaka Banno, Kohji Yamamoto

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

Kunitz-type chymotrypsin inhibitor CIb1 of silkworm Bombyx mori is a basic peptide consisting of 62 amino acid residues. To elucidate the mechanisms of transcriptional regulation of CIb1 gene expression, we cloned it for genomic structure analysis. CIb1 cDNA was used as a probe to screen a BAC sub-library. One positive clone containing the upstream sequences was isolated and the sequence result showed that CIb1 gene consists of three exons spaced by two introns. In the 5′-flanking region, consensus TATA and CCAAT boxes were identified. Other binding sites for transcription factors such as NF-κB, GATA, C/EBP, COUP-TF/HNF-4, RORα1, SRY, and HOXA3 were also detected. Southern blot analysis suggested a single copy of CIb1 gene in the silkworm genome. Northern blot analysis indicated that the expression of CIb1 gene is transcriptionally regulated during development and is apparently tissue-specific. The CIb1 mRNA was detected in fat body, ovary, trachea, and skin. We furthermore investigated the CIb1 expression profiles after LPS and E. coli injection. The fluctuations of CIb1 transcript in challenged larvae confirm our proposal that CIb1 is an immune responsible gene. According to our data, we discussed the transcriptional factors putatively responsible for the physiological role of CIb1 in the silkworm hemolymph.

元の言語英語
ページ(範囲)71-77
ページ数7
ジャーナルBiochimica et Biophysica Acta - Gene Structure and Expression
1628
発行部数1
DOI
出版物ステータス出版済み - 7 9 2003

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Gene encoding
Bombyx
Chymotrypsin
Genes
Gene Expression
TATA Box
Fat Body
Hemolymph
5' Flanking Region
Gene Expression Regulation
Southern Blotting
Trachea
Northern Blotting
Introns
Libraries
Larva
Ovary
Exons
Transcription Factors
Gene expression

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

これを引用

Genomic structure and expression analysis of the gene encoding a silkworm basic Kunitz-type chymotrypsin inhibitor. / He, Ningjia; Yakiyama, Masatoshi; Fujii, Hiroshi; Banno, Yutaka; Yamamoto, Kohji.

:: Biochimica et Biophysica Acta - Gene Structure and Expression, 巻 1628, 番号 1, 09.07.2003, p. 71-77.

研究成果: ジャーナルへの寄稿記事

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