抄録
Histones are basic protein monomers capable of interacting with DNA, providing the mechanism of DNA compaction inside the cell nucleus. The well-ordered assembly process of histone and DNA is a potential candidate as the approach for building DNA-protein nanostructures. Here, utilizing the sequence-independent histone-DNA interaction, we present an approach to self-assemble histones and single-stranded DNA (ssDNA) to form well-defined histone-DNA (sHD) nanoparticles and their multidimensional cross-linked complexes (cHD). By using various molecular biology and microscopy techniques, we elucidate the structure of these complexes, and we show that they are formed at carefully controlled conditions of temperature, ionic strength, concentration, and incubation time. We also demonstrate using a set of ssDNA molecular rulers and a geometric accommodation model that the assembly of sHD and cHD particles proceeds with precise geometry so that the number of ssDNA in these particles can be programmed by the length of ssDNA. We further show that the formation of cHD amplifies the effect of the length of ssDNA on the self-assembly, allowing for distinguishing ssDNA of different lengths at single nucleotide resolution. We envision that our geometry-directed approach of self-assembling histone-DNA nanostructures and the fundamental insights can serve as a structural platform to advance building precisely ordered DNA-protein nanostructures.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 8155-8168 |
| ページ数 | 14 |
| ジャーナル | ACS nano |
| 巻 | 13 |
| 発行部数 | 7 |
| DOI | |
| 出版物ステータス | 出版済み - 7 23 2019 |
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All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Engineering(all)
- Physics and Astronomy(all)
これを引用
Geometry-Based Self-Assembly of Histone-DNA Nanostructures at Single-Nucleotide Resolution. / Serag, Maged F.; Aikeremu, Aimaiti; Tsukamoto, Ryoko; Piwoński, Hubert; Abadi, Maram; Kaji, Noritada; Dwyer, Jason R.; Baba, Yoshinobu; Habuchi, Satoshi.
：: ACS nano, 巻 13, 番号 7, 23.07.2019, p. 8155-8168.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Geometry-Based Self-Assembly of Histone-DNA Nanostructures at Single-Nucleotide Resolution
AU - Serag, Maged F.
AU - Aikeremu, Aimaiti
AU - Tsukamoto, Ryoko
AU - Piwoński, Hubert
AU - Abadi, Maram
AU - Kaji, Noritada
AU - Dwyer, Jason R.
AU - Baba, Yoshinobu
AU - Habuchi, Satoshi
PY - 2019/7/23
Y1 - 2019/7/23
N2 - Histones are basic protein monomers capable of interacting with DNA, providing the mechanism of DNA compaction inside the cell nucleus. The well-ordered assembly process of histone and DNA is a potential candidate as the approach for building DNA-protein nanostructures. Here, utilizing the sequence-independent histone-DNA interaction, we present an approach to self-assemble histones and single-stranded DNA (ssDNA) to form well-defined histone-DNA (sHD) nanoparticles and their multidimensional cross-linked complexes (cHD). By using various molecular biology and microscopy techniques, we elucidate the structure of these complexes, and we show that they are formed at carefully controlled conditions of temperature, ionic strength, concentration, and incubation time. We also demonstrate using a set of ssDNA molecular rulers and a geometric accommodation model that the assembly of sHD and cHD particles proceeds with precise geometry so that the number of ssDNA in these particles can be programmed by the length of ssDNA. We further show that the formation of cHD amplifies the effect of the length of ssDNA on the self-assembly, allowing for distinguishing ssDNA of different lengths at single nucleotide resolution. We envision that our geometry-directed approach of self-assembling histone-DNA nanostructures and the fundamental insights can serve as a structural platform to advance building precisely ordered DNA-protein nanostructures.
AB - Histones are basic protein monomers capable of interacting with DNA, providing the mechanism of DNA compaction inside the cell nucleus. The well-ordered assembly process of histone and DNA is a potential candidate as the approach for building DNA-protein nanostructures. Here, utilizing the sequence-independent histone-DNA interaction, we present an approach to self-assemble histones and single-stranded DNA (ssDNA) to form well-defined histone-DNA (sHD) nanoparticles and their multidimensional cross-linked complexes (cHD). By using various molecular biology and microscopy techniques, we elucidate the structure of these complexes, and we show that they are formed at carefully controlled conditions of temperature, ionic strength, concentration, and incubation time. We also demonstrate using a set of ssDNA molecular rulers and a geometric accommodation model that the assembly of sHD and cHD particles proceeds with precise geometry so that the number of ssDNA in these particles can be programmed by the length of ssDNA. We further show that the formation of cHD amplifies the effect of the length of ssDNA on the self-assembly, allowing for distinguishing ssDNA of different lengths at single nucleotide resolution. We envision that our geometry-directed approach of self-assembling histone-DNA nanostructures and the fundamental insights can serve as a structural platform to advance building precisely ordered DNA-protein nanostructures.
UR - http://www.scopus.com/inward/record.url?scp=85070485879&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85070485879&partnerID=8YFLogxK
U2 - 10.1021/acsnano.9b03259
DO - 10.1021/acsnano.9b03259
M3 - Article
C2 - 31244030
AN - SCOPUS:85070485879
VL - 13
SP - 8155
EP - 8168
JO - ACS Nano
JF - ACS Nano
SN - 1936-0851
IS - 7
ER -