Germ cell-less like-2 protein is a new component of outer dense fibers in rat sperm flagella

Emi Murayama, Michiko Katoh, Akina Kanebayashi, Takane Kaneko, Yosaburo Shibata, Tetsuichiro Inai, Hiroshi Iida

研究成果: Contribution to journalArticle査読

4 被引用数 (Scopus)

抄録

We have analyzed the expression profiles of ten genes in terms of testis development and organ specificity in rat, which were selected from 215 round spermatid-specific transcripts listed in a database. Out of the ten genes, we directed our attention to one gene, a germ cell-less like-2 gene (gcl-2), a homolog of Drosophila gcl gene (gcl), which is a component of the germ plasma and required for primordial germ cell formation. Rat genome contains duplicate rat gcl-2 (rgcl-2) genes, rgcl-2A and rgcl-2B, both of which are located at Xq13. RT-PCR analysis showed that the expression of the two genes was up-regulated during testis development and that they were predominantly expressed in the testis. Both rgcl-2A and rgcl-2B encode a protein of 498 amino acid residues, showing 90.56% identity at the amino acid level. Confocal laser scanning microscopy revealed that rgcl-2 protein was synthesized in the cytoplasm of elongating spermatids and at least a part of it was integrated into the middle piece of spermatozoa during spermiogenesis. Immunogold electron microscopy uncovered that rgcl-2 was localized at the abaxial (convex) surface of outer dense fibers (ODF) of rat sperm flagella. Therefore, we concluded that rgcl-2 is a new component of ODF in sperm flagella.

本文言語英語
ページ(範囲)749-756
ページ数8
ジャーナルReproduction
134
6
DOI
出版ステータス出版済み - 12 2007

All Science Journal Classification (ASJC) codes

  • 生殖医学
  • 胎生学
  • 内分泌学
  • 産婦人科学
  • 細胞生物学

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