Germinal vesicle materials are not required for the activation of MAP kinase in porcine oocyte maturation

K. Sugiura, K. Naito, N. Iwamori, H. Kagii, S. Goto, S. Ohashi, K. Yamanouchi, H. Tojo

研究成果: Contribution to journalArticle査読

18 被引用数 (Scopus)

抄録

The requirement of the germinal vesicle (GV) for the normal kinetics of mitogen-activated protein (MAP) kinase activity during porcine oocyte maturation was investigated. Porcine follicular oocytes were enucleated, and the locations of their extracellular signal-regulated kinases 1 and 2 (ERK1/2), major MAP kinases in maturating porcine oocytes, were detected by indirect immunofluorescent microscopy. The MAP kinase activity was assayed as myelin basic protein (MBP) kinase activity, and the phosphorylation states of ERK1/2 were detected by immunoblotting analyses. Translocation of MAP kinase into the GV and association with the spindle were observed in intact oocytes, while MAP kinase in enucleated oocytes was distributed almost uniformly in cytoplasm throughout the culturing period. The phosphorylation and the activation of MAP kinase were induced, and the activity was comparable with that of control denuded oocytes. The high level of activity was maintained through maturation, even in the absence of spindle formation. These results indicate that the presence of nuclear material and translocation into the GV are dispensable for the activation of MAP kinase and that associating with the spindle is not required for maintenance of its activity though porcine oocyte maturation.

本文言語英語
ページ(範囲)215-220
ページ数6
ジャーナルMolecular Reproduction and Development
59
2
DOI
出版ステータス出版済み - 2001
外部発表はい

All Science Journal Classification (ASJC) codes

  • 遺伝学
  • 発生生物学
  • 細胞生物学

フィンガープリント

「Germinal vesicle materials are not required for the activation of MAP kinase in porcine oocyte maturation」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル