Ght2+ is required for UDP-galactose synthesis from extracellular galactose by Schizosaccharomyces pombe

Tomohiko Matsuzawa, Futoshi Hara, Naotaka Tanaka, Hideki Tohda, Kaoru Takegawa

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

Schizosaccharomyces pombe has eight hexose transporter genes, ght1 + to ght8 +. Here we report that ght2 +, which is highly expressed in the presence of glucose, is essential for UDP-galactose synthesis from extracellular galactose when cells grow on glucose. The galactosylation defect of a uge1Δ mutant defective in synthesis of UDP-galactose from glucose was suppressed in galactose-containing medium, but disruption of ght2 + in the uge1Δ mutant reversed suppression of the galactosylation defect. Expression of Saccharomyces cerevisiae GAL2 in uge1Δght2Δ cells suppressed the defective galactosylation phenotype in galactose-containing medium. These results indicate that galactose is transported from the medium to the cytosol in a Ght2-dependent manner, and is then converted into UDP-galactose.

本文言語英語
ページ(範囲)4957-4964
ページ数8
ジャーナルApplied Microbiology and Biotechnology
97
11
DOI
出版ステータス出版済み - 6 1 2013

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

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