Global regulation by horizontally transferred regulators establishes the pathogenicity of escherichia coli

Hiroyuki Abe, Akira Miyahara, Taku Oshima, Kosuke Tashiro, Yoshitoshi Ogura, Satoru Kuhara, Naotake Ogasawara, Tetsuya Hayashi, Toru Tobe

研究成果: ジャーナルへの寄稿学術誌査読

44 被引用数 (Scopus)

抄録

Enterohemorrhagic Escherichia coli is an emerging pathogen that causes diarrhea and hemolytic uremic syndrome. Much of the genomic information that affects virulence is acquired by horizontal transfer. Genes necessary for attaching and effacing lesions are located in the locus for enterocyte effacement (LEE) pathogenicity island. LEE gene transcription is positively regulated by Ler, which is also encoded by the LEE, and by Pch regulators, which are encoded at other loci. Here we identified genes whose transcription profiles were similar to those of the LEE genes, by comparing the effects of altering ler and pch transcript levels. We assigned these genes into two classes, according to their transcription profiles. By determining the binding profiles for Ler and Pch, we showed that both were involved in regulating one class of genes, but only Pch was involved in regulating the other class. Binding sites were found in the coding region as well as the promoter region of regulated genes, which include genes common to K12 strains as well as 0157-specific genes, suggesting that both act as a global regulator. These results indicate that Ler and Pch orchestrate the transcription of virulence genes, which are captured by horizontal transfer and scattered throughout the chromosome.

本文言語英語
ページ(範囲)25-38
ページ数14
ジャーナルDNA Research
15
1
DOI
出版ステータス出版済み - 2008

!!!All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

フィンガープリント

「Global regulation by horizontally transferred regulators establishes the pathogenicity of escherichia coli」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル