TY - JOUR
T1 - Global transcriptional and circadian regulation in a halotolerant cyanobacterium Halothece sp. PCC7418
AU - Waditee-Sirisattha, Rungaroon
AU - Ito, Hiroshi
AU - Kageyama, Hakuto
N1 - Funding Information:
We thank Professor Takao Kondo (Nagoya University) for the generous gift of anti-KaiC antibody, and members of the laboratories for fruitful discussions. This work was supported in part by the research fund of Mayekawa Houonkai Foundation A2-20005 (to H.K.), the research support fund of the Research Institute of Meijo University (to H.K.), Japan Society for the Promotion of Science KAKENHI Grants 18H05474 (to H.I.), and Thailand Science Research and Innovation Fund, Chulalongkorn University (CUFRB65_hea(67)_130_23_60) (to R.W.S.).
Funding Information:
We thank Professor Takao Kondo (Nagoya University) for the generous gift of anti-KaiC antibody, and members of the laboratories for fruitful discussions. This work was supported in part by the research fund of Mayekawa Houonkai Foundation A2-20005 (to H.K.), the research support fund of the Research Institute of Meijo University (to H.K.), Japan Society for the Promotion of Science KAKENHI Grants 18H05474 (to H.I.), and Thailand Science Research and Innovation Fund, Chulalongkorn University (CUFRB65_hea(67)_130_23_60) (to R.W.S.).
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Substantial evidence has been accumulated about the molecular basis underlying halotolerance; however, insights into the regulatory networks for relevant genes and mechanisms of their interplay remain elusive. Here, we present a comprehensive transcriptome investigation, using RNA sequencing, of specific metabolic pathways and networks in a halotolerant cyanobacterium, Halothece sp. PCC7418, including the circadian rhythm profile. Dissecting the transcriptome presented the intracellular regulation of gene expressions, which was linked with ion homeostasis, protein homeostasis, biosynthesis of compatible solutes, and signal transduction, for adaptations to high-salinity environments. The efficient production and distribution of energy were also implicated in this acclimation process. Furthermore, we found that high-salinity environments had a dramatic effect on the global transcriptional expression regulated by the circadian clock. Our findings can provide a comprehensive transcriptome for elucidating the molecular mechanisms underlying halotolerance in cyanobacteria.
AB - Substantial evidence has been accumulated about the molecular basis underlying halotolerance; however, insights into the regulatory networks for relevant genes and mechanisms of their interplay remain elusive. Here, we present a comprehensive transcriptome investigation, using RNA sequencing, of specific metabolic pathways and networks in a halotolerant cyanobacterium, Halothece sp. PCC7418, including the circadian rhythm profile. Dissecting the transcriptome presented the intracellular regulation of gene expressions, which was linked with ion homeostasis, protein homeostasis, biosynthesis of compatible solutes, and signal transduction, for adaptations to high-salinity environments. The efficient production and distribution of energy were also implicated in this acclimation process. Furthermore, we found that high-salinity environments had a dramatic effect on the global transcriptional expression regulated by the circadian clock. Our findings can provide a comprehensive transcriptome for elucidating the molecular mechanisms underlying halotolerance in cyanobacteria.
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U2 - 10.1038/s41598-022-17406-6
DO - 10.1038/s41598-022-17406-6
M3 - Article
C2 - 35962002
AN - SCOPUS:85135971297
SN - 2045-2322
VL - 12
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 13190
ER -