Glucose reduced PDGF production and cell proliferation of cultured vascular endothelial cells

F. Umeda, T. Yamauchi, N. Nakashima, H. Ono, H. Nawata, M. Masuko, K. Nakayama, A. Tatematsu

研究成果: Contribution to journalArticle

8 引用 (Scopus)

抜粋

The effect of glucose on PDGF production and cell proliferation was studied on cultured bovine aortic endothelial cells. PDGF levels were measured using an enzyme-linked immunosorbent assay technique newly developed in our laboratory. The cell proliferation rate was determined on the basis of 3H-thymidine incorporation into cellular DNA. PDGF levels in culture medium were below the detection limit of the assay. However, PDGF levels were measurable in cultured endothelial cells at confluence. Both PDGF production and thymidine incorporation were significantly reduced in the endothelial cells cultured with high concentrations of glucose. These results suggest that reduced PDGF production and cell proliferation may be involved in altered vascular endothelial function in diabetics.

元の言語英語
ページ(範囲)274-277
ページ数4
ジャーナルHormone and Metabolic Research
23
発行部数6
DOI
出版物ステータス出版済み - 1 1 1991

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

フィンガープリント Glucose reduced PDGF production and cell proliferation of cultured vascular endothelial cells' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用