Glycoinsulins: Dendritic sialyloligosaccharide-displaying insulins showing a prolonged blood-sugar-lowering activity

Masaaki Sato, Tetsuya Furuike, Reiko Sadamoto, Naoki Fujitani, Taku Nakahara, Kenichi Niikura, Kenji Monde, Hirosato Kondo, Shin Ichiro Nishimura

研究成果: Contribution to journalArticle査読

68 被引用数 (Scopus)

抄録

Mono-, di-, and trisialyloligosaccharides were introduced to mutant insulins through enzymatic reactions. Sugar chains were sialylated by α2,6-sialyltransferase (α2,6-SiaT) via an accessible glutamine residue at the N-terminus of the B-chain attached by transglutaminase (TGase). Sia2,6-di-LacNAc-Ins(B-F1Q) and Sia2,6-tri-LacNAc-Ins(B-F1Q), displaying two and three sialyl-N-acetyllactosamines, respectively, were administered to hyperglycemic mice. Both branched glycoinsulins showed prolonged glucose-lowering effects compared to native or lactose-carrying insulins, showing that sialic acid is important in obtaining a prolonged effect. Sia2,6-tri-LacNAc-Ins(B-F1Q), in particular, induced a significant delay in the recovery of glucose levels.

本文言語英語
ページ(範囲)14013-14022
ページ数10
ジャーナルJournal of the American Chemical Society
126
43
DOI
出版ステータス出版済み - 11 3 2004
外部発表はい

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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