Glycolytic enzyme Pgk1 is strongly expressed in the developing tooth germ of the mouse lower first molar

Jun ya Honda, Ieyoshi Kobayashi, Tamotsu Kiyoshima, Haruyoshi Yamaza, Ming Xie, Keiko Takahashi, Norio Enoki, Kengo Nagata, Akihiko Nakashima, Hidetaka Sakai

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抄録

This study examined detailed in situ expression patterns and possible functional roles of phosphoglycerate kinase 1 (Pgk1) gene in the developing tooth germ of the mouse lower first molar. The strong expression of Pgk1 mRNA was seen in the odontogenic epithelial cells and surrounding mesenchymal cells of the tooth germ from embryonic day 10.5 (E10.5) to E18.0. Western blotting analysis demonstrated that Pgk1 protein formed 84-kDa protein complex in these embryonic organs. The results of immunoprecipitation-western blotting also suggested this complex to be formed with glyceraldehyde-3- phosphate dehydrogenase (GAPDH). Moreover, the immunofluorescence expression of those proteins was shown to overlap each other in the tooth germ at E15.0. A strong immunofluorescence expression of both Pgk1 and GAPDH also corresponded to the in situ expression of those mRNAs. These results suggested that Pgk1 plays some functional roles in the development of tooth germ and other embryonic organs by forming protein complex with GAPDH.

元の言語英語
ページ(範囲)423-432
ページ数10
ジャーナルHistology and Histopathology
23
発行部数4
出版物ステータス出版済み - 4 2008

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Tooth Germ
Phosphoglycerate Kinase
Glyceraldehyde-3-Phosphate Dehydrogenases
Enzymes
Fluorescent Antibody Technique
Proteins
Western Blotting
Messenger RNA
Immunoprecipitation
Epithelial Cells
Genes

All Science Journal Classification (ASJC) codes

  • Histology
  • Pathology and Forensic Medicine

これを引用

Glycolytic enzyme Pgk1 is strongly expressed in the developing tooth germ of the mouse lower first molar. / Honda, Jun ya; Kobayashi, Ieyoshi; Kiyoshima, Tamotsu; Yamaza, Haruyoshi; Xie, Ming; Takahashi, Keiko; Enoki, Norio; Nagata, Kengo; Nakashima, Akihiko; Sakai, Hidetaka.

:: Histology and Histopathology, 巻 23, 番号 4, 04.2008, p. 423-432.

研究成果: ジャーナルへの寄稿記事

Honda, JY, Kobayashi, I, Kiyoshima, T, Yamaza, H, Xie, M, Takahashi, K, Enoki, N, Nagata, K, Nakashima, A & Sakai, H 2008, 'Glycolytic enzyme Pgk1 is strongly expressed in the developing tooth germ of the mouse lower first molar', Histology and Histopathology, 巻. 23, 番号 4, pp. 423-432.
Honda, Jun ya ; Kobayashi, Ieyoshi ; Kiyoshima, Tamotsu ; Yamaza, Haruyoshi ; Xie, Ming ; Takahashi, Keiko ; Enoki, Norio ; Nagata, Kengo ; Nakashima, Akihiko ; Sakai, Hidetaka. / Glycolytic enzyme Pgk1 is strongly expressed in the developing tooth germ of the mouse lower first molar. :: Histology and Histopathology. 2008 ; 巻 23, 番号 4. pp. 423-432.
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abstract = "This study examined detailed in situ expression patterns and possible functional roles of phosphoglycerate kinase 1 (Pgk1) gene in the developing tooth germ of the mouse lower first molar. The strong expression of Pgk1 mRNA was seen in the odontogenic epithelial cells and surrounding mesenchymal cells of the tooth germ from embryonic day 10.5 (E10.5) to E18.0. Western blotting analysis demonstrated that Pgk1 protein formed 84-kDa protein complex in these embryonic organs. The results of immunoprecipitation-western blotting also suggested this complex to be formed with glyceraldehyde-3- phosphate dehydrogenase (GAPDH). Moreover, the immunofluorescence expression of those proteins was shown to overlap each other in the tooth germ at E15.0. A strong immunofluorescence expression of both Pgk1 and GAPDH also corresponded to the in situ expression of those mRNAs. These results suggested that Pgk1 plays some functional roles in the development of tooth germ and other embryonic organs by forming protein complex with GAPDH.",
author = "Honda, {Jun ya} and Ieyoshi Kobayashi and Tamotsu Kiyoshima and Haruyoshi Yamaza and Ming Xie and Keiko Takahashi and Norio Enoki and Kengo Nagata and Akihiko Nakashima and Hidetaka Sakai",
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AU - Honda, Jun ya

AU - Kobayashi, Ieyoshi

AU - Kiyoshima, Tamotsu

AU - Yamaza, Haruyoshi

AU - Xie, Ming

AU - Takahashi, Keiko

AU - Enoki, Norio

AU - Nagata, Kengo

AU - Nakashima, Akihiko

AU - Sakai, Hidetaka

PY - 2008/4

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N2 - This study examined detailed in situ expression patterns and possible functional roles of phosphoglycerate kinase 1 (Pgk1) gene in the developing tooth germ of the mouse lower first molar. The strong expression of Pgk1 mRNA was seen in the odontogenic epithelial cells and surrounding mesenchymal cells of the tooth germ from embryonic day 10.5 (E10.5) to E18.0. Western blotting analysis demonstrated that Pgk1 protein formed 84-kDa protein complex in these embryonic organs. The results of immunoprecipitation-western blotting also suggested this complex to be formed with glyceraldehyde-3- phosphate dehydrogenase (GAPDH). Moreover, the immunofluorescence expression of those proteins was shown to overlap each other in the tooth germ at E15.0. A strong immunofluorescence expression of both Pgk1 and GAPDH also corresponded to the in situ expression of those mRNAs. These results suggested that Pgk1 plays some functional roles in the development of tooth germ and other embryonic organs by forming protein complex with GAPDH.

AB - This study examined detailed in situ expression patterns and possible functional roles of phosphoglycerate kinase 1 (Pgk1) gene in the developing tooth germ of the mouse lower first molar. The strong expression of Pgk1 mRNA was seen in the odontogenic epithelial cells and surrounding mesenchymal cells of the tooth germ from embryonic day 10.5 (E10.5) to E18.0. Western blotting analysis demonstrated that Pgk1 protein formed 84-kDa protein complex in these embryonic organs. The results of immunoprecipitation-western blotting also suggested this complex to be formed with glyceraldehyde-3- phosphate dehydrogenase (GAPDH). Moreover, the immunofluorescence expression of those proteins was shown to overlap each other in the tooth germ at E15.0. A strong immunofluorescence expression of both Pgk1 and GAPDH also corresponded to the in situ expression of those mRNAs. These results suggested that Pgk1 plays some functional roles in the development of tooth germ and other embryonic organs by forming protein complex with GAPDH.

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